The genetic variation of Indonesian native chicken crossbred between Putih and Wareng chicken based on PRL gene exon 4

¹ Animal Science Division, Center for Biotechnology Development, University of Muhammadiyah Malang, Indonesia 65145
² Deapartment of Animal Sciences, Faculty of Agriculture and Animal Science, University of Muhammadiyah Malang, Indonesia 65145

^* Corresponding author: winaya@umm.ac.id

Abstract

The PRL gene in chickens is one gene that plays a role in hatchability and egg production. Therefore, knowing the variation of the PRL gene can be used to predict the performance of egg productivity of native chickens. This study observed the genetics of Indonesian native chickens, White and Wareng chickens and their crossbred based on the Prolactin (PRL) gene in exon 4. Chicken samples were kept at the Experimental Farm, Faculty of Agriculture and Animal Science, University of Muhammadiyah Malang (UMM), Indonesia. DNA isolation and PCR reactions were carried out at the UMM Biotechnology Development Laboratory. Meanwhile, PRL gene exon four sequencing was carried out at the Macrogen Asia Pacific Pte. Ltd Laboratory, Singapore. Gene sequence analysis showed that local Indonesian Putih and Wareng chickens and their crosses were closely related to Gallus gallus and Gallus gallus strain White Leghorn based on the phylogenetic tree with a bootstrap value of 91%. The average genetic distance between native chicken samples was 0.036 and the Tajima test was not significant (p> 0.01) with a value of -1.35379. For further research, it is necessary to research the correlation between gene variation and egg production performance in Indonesian native chickens and their crossbreeds.