Potato microclonal propagation method in a temporary immersion bioreactor

Siberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of Sciences, Novosibirsk region, Novosibirsk district, Krasnoobsk village, Russia, 633501

^* Corresponding author: sibime@yandex.ru

Abstract

A promising and efficient approach to obtaining a genetically pure and identical plant to a given explant is through microclonal propagation. This method pre-determines the reproduction of plants by managing, controlling, and regulating the parameters of external and internal conditions that influence the accelerated reproduction of cultivated plants. The main methods for implementing the accelerated reproduction of cultivated plants are propagation in a closed external environment using specialized nutrient solutions and the use of bioreactors with temporary immersion of plants. The process of reproducing cultivated plants, specifically potatoes, using temporary immersion bioreactors allows for the use of automated monitoring and control systems based on robust control systems. This increases productivity and ensures the quality of the reproduced explants. Research has led to the development of experienced statisticians who have established the impact of individual parameters on the management of plant reproduction on their quality indicators. The reliability of the study is ensured by a large sample size (over 200 objects), parametric statistics, and the use of group theory to estimate plant reproduction parameters.