Isolated culture of A. reptance L., its’ morphological and growth features

¹ Department of Biochemistry, Microbiology & Biotechnology, Biology Faculty, Yerevan State University, 0025 Yerevan, Armenia
² Research Institute of Biology, Yerevan State University, 0025 Yerevan, Armenia
³ Institute of Pharmacy, Chemistry and Biology, Belgorod State National Research University, 308015, Belgorod, Russian Federation

^* Corresponding author: k.trchounian@ysu.am

Abstract

A growing demand for the ecologically pure products brings us for searching novel biotechnological approaches for plant cultivation. One of these approaches is the in vitro cultivation and further acclimatization of valuable plant species. The object of our investigation was Ajugareptance L. ornamental plant which possesses high metabolic activity. In vitro cultivation was carried out applying Murashige-Skoog nutrient medium and its modifications. Acclimatization of in vitro plants was implemented according Hazarika. In the presence of twice higher concentration of cytokinins over auxins and 0.2 mg/ml gibberellins callus culture was formed from the leaf explants. Callus tissue was formed in the presence of 0.2 mg/ml kinetin and 2 mg/ml indole-3-acetic acid which has denser structure than the first one. The shoot formation was observed on callus cultures growing on the same medium approximately after 5^th passage. Callus culture growth was supported also by the adding of 2 mg/ml 2,4-dichlorophenoxyacetic acid. For the micropropagation, the already formed shoots were transferred to the nutrient medium which contains only 0.1 mg/ml 1-Naphthaleneacetic acid as a phytohormone. A. reptans culture has high regenerative ability and the micro-propagation index was 10⁴ – 10⁵. In vitro regenerated plants were successfully acclimatized to the soil conditions during two-week period.