Antibacterial Activity of Magnesium Oxide Nanoparticles against MDR Pseudomonas aeruginosa Isolated from Different Clinical Infections

University of Kufa, Kufa, Iraq

^* Corresponding author: shhablshf@gmail.com

Abstract

In this study, 180 isolates of Pseudomonas aeruginosa were isolated from patients suffering from various infections, including urinary tract infections, burns, ear infections, tonsillitis, and pneumonia. Specimens were taken from patients hospitalized in Al-Hakim General Hospital, Al-Sadr Medical City, and the Burn Center in Najaf Al-Ashraf, and they were transferred to the Microbiology Laboratory in the Biology Department in the College of Science. P. aeruginosa have been isolated from Specimens taken from patients with these various infections. Isolation methods were different, including culture on MacConkey medium, blood agar, as well as various biochemical and Vitek tests. Susceptibility testing was also performed on these bacteria for six families according to CLSI. These families are penicillins, aminoglycosides, carbopenems, cephalosporins, fluoroquinins, and lipopeptides. It was noted that forty isolates were multi-resistant to these antibiotics. It was also revealed that resistant isolates formed biofilms using a flat microliter. The effectiveness of domestic and imported magnesium oxide nanoparticles on resistant isolates was conducted. These two types were taken at concentrations of 100, 150, and 200 μg/ml. Imported MgO nanoparticles were more effective than domestic ones. It was also observed that the effect of magnesium oxide nanoparticles on resistant bacteria increased with increasing concentration. Antibiotics resistant to Pseudomonas aeruginosa bacteria were also taken and mixed with imported and local magnesium oxide nanoparticles with an optimal concentration of (200) μg/ml. The effect of magnesium oxide nanoparticles combined with antibiotics was greater than if the nanoparticles were alone.