In vitro development of preimplantation cattle embryos obtained from oocytes irradiated with low doses of ionizing radiation

Federal State Budgetary Scientific Institution Ural Federal Agrarian Scientific Research Centre, Ural Branch of the Russian Academy of Sciences (FSBSI UrFASRC, UrB of RAS), 112 А, Belinskogo St., 620000, Еkaterinburg, Russia

^* Corresponding author: isaeva.05@bk.ru

Abstract

Radiation-induced processes in germ reproductive cells, as well as the effect of low doses of radiation on the reproductive potential of farm animals remain poorly studied. Assessing the radiation effects of low doses on gametes and early embryos will reveal biological features that are important for assisted reproductive technologies in animal husbandry. In addition, the study of the processes of maturation and fertilization of irradiated gametes and the subsequent development of early preimplantation embryos will make it possible to update measures to protect the health and reproductive potential of farm animals in conditions of technogenic radiation risks. In this study, we focused on analyzing the effects of irradiation on the ovaries of cattle and examining the consequences of this exposure on embryo development under in vitro fertilization conditions. We used X-ray radiation of different dose rates with an absorbed dose of 100 mGy. The research results showed a decrease in the frequency of blastocyst formation in all experimental groups of embryos obtained from oocytes of irradiated ovaries. The data obtained can serve as a scientific basis for the development of measures to preserve the reproductive potential of farm animals under technogenic radiation risks, as well as for further search of ways to improve assisted reproductive technologies in animal husbandry.