Effects of Exopolysaccharides on the regulation of FTH1 protein expression and cell death of HaCaT cells under ultraviolet irradiation

Emory University, Address: 201 Dowman Dr, Atlanta, GA 30322

Email: ruoxi.wu115@gmail.com

Abstract

Previous research works indicate that natural exopolysaccharide (EPS) produced by lactic acid bacteria Lactobacillus plantarum HY7714 might be effective at anti-photoaging and due to its function as antioxidants against reactive oxygen species (ROS). Through regulating the expression level of FTH1, intracellular iron dyshomeostasis induced by UV irradiation might be relieved. This research paper investigates on the regulation of exopolysaccharides on FTH1 expression, ferritin concentration and level of lipid peroxidation in HaCaT cells under UVA+UVB exposure. In our research, we found that HaCaT cell viability is higher when treated with exopolysaccharides, which indicates that exopolysaccharides could effectively improve cell viability under UV radiation. Also, the presence of EPS and FTH1 are shown to decrease lipid peroxidation level and reduce intracellular ferritin concentration, which demonstrates that EPS could upregulate the FTH1 expression level to mediate the ferritin concentration in the HaCaT cells which would further balance iron homeostasis. Ultimately, EPS are proved to decrease cell death under UV radiation through modulating FTH1 protein expression.