Study of regime parameters of the process of cultivation of a consortium of probiotic microorganisms providing increased synthesis of exopolysaccharides

Voronezh State University of Engineering Technologies, 19, Revolution Avenue, Voronezh, 394036, Russia

^* Corresponding author: e_s_popov@mail.ru

Abstract

The paper conducted experimental studies of the activity of exopolysaccharide synthesis by a consortium of probiotic microorganisms based on Streptococcus thermophiles, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium adolescentis, Bifidobacterium breve, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus fermentum. Temperature conditions of cultivation were studied on the basis of combination of stages of optimal and non-optimal conditions for development of microorganisms corresponding to temperature variation in the range of higher or lower values, in order to intensify synthesis of moisture-binding metabolites of polysaccharide nature. The results of studies of optical density and concentration of biomass exopolysaccharides are given, which indicate a positive effect on the synthesis of exopolysaccharides of the used combined temperature modes of cultivation. Based on thermal analysis data, endothermic effects were identified, indicating an additional increase in the degree of moisture binding by polysaccharides of probiotic origin. It was found that the maximum activity of the synthesis of moisture-binding metabolites corresponds to the combined cultivation mode with a sequential transition from the area of low temperature effects to the optimal area.