Influence of nutrition medium composition on biosynthesis of agglutinins of Rhizoctonia solani RS

¹ Federal Center for Toxicological, Radiation and Biological Safety, Laboratory of Cell Cultures and Nutrient Media, 420075 Kazan, Russia
² All-Russian Research Institute of Phytopathology, Laboratory of biologically active substances, 143050 Odintsovo, Russia
³ Kazan (Volga Region) Federal University, Department of biochemistry, biotechnology and pharmacology, 420008 Kazan, Russia

^* Corresponding author: lrvaliullin@yandex.ru

Abstract

The influence of the composition of the nutrient medium on the yield of biomass and the biosynthesis of agglutinins of the mold fungus strain Rhizoctonia solani RS was studied. At the first stage of research, the agglutinin-producing micromycete strain was grown on liquid nutrient media (potato-glucose (PG), Chapek, Sabouraud) at a temperature of 28°C for 8 days. At the next stage of research, the composition of the culture medium was modified in order to select sources of nitrogen (NH₄NO₃, (NH₄)₂SO₄, NaNO₃, arginine, tryptophan, asparagine, threonine, peptone) and carbon (sucrose, glucose, starch) nutrition. Cultivation of the Rh. solani RS on a liquid PG medium, containing glucose in an amount of 20.0 g/l led to a maximum yield of fungal biomass of 23.58 ± 1.30 g/l and the production of its agglutinins (titer 16384). The studied micromycete strain was characterized by the ability to actively use the amino acids threonine or asparagine when added to the above nutrient medium in an amount of 0.1 mg/ml. At the same time, the hemagglutination activity of the micromycete agglutinins increased twofold (titer 32768) compared to the activity of agglutinins on the PG medium without the addition of amino acids (titer 16384).