Increasing the productivity of hydrolase producers by induced mutagenesis and their comparative characteristics

¹ All-Russian Research Institute of Phytopathology, Laboratory of biologically active substances, 143050 Odintsovo, Russia
² Federal Center for Toxicological, Radiation and Biological Safety, Laboratory of Cell Cultures and Nutrient Media, 420075 Kazan, Russia

^* Corresponding author: tanirtashir@mail.ru

Abstract

In order to study the increase in productivity of natural hydrolase producers B. subtilis (producer of amylolytic and proteolytic enzymes) and T. harzianum (producer of xylolytic and cellulolytic enzymes), the efficiency of various approaches to non-directed induced mutagenesis was studied and their comparative characteristics were carried out. Using chemical and physical mutagenesis, new highly active producers of extracellular hydrolytic enzymes B. subtilis strain MB085-1457, B. subtilis strain MB085-2701, B. subtilis strain MB085-3689, T. harzianum strain MF091-962, T. harzianum strain MF091-2093 and T. harzianum strain MF091-3416 were obtained. Comparison of various methods of induced mutagenesis, namely methods of mutagenesis using nitrosoguanidine and nitrosomethylurea, UV and gamma irradiation showed that the greatest efficiency was achieved after mutagenesis using gamma rays. Various methods of induced mutagenesis can be used not only to create effective schemes for increasing the level of accumulation of target enzymes of producers, but also to obtain new drugs based on highly productive strains of microorganisms with hydrolase activity for the purpose of using them in resource-saving technologies for processing agricultural waste (into useful resources) and reducing the negative impact on the environment.