Obtaining stable strains of microorganisms with increased production of hydrolytic enzymes by methods of induced mutagenesis and their characteristics

¹ Federal Center for Toxicological, Radiation and Biological Safety, 2 Nauchny Gorodok, Kazan, 420075, Russia
² State Scientific Institution “All-Russian Research Institute of Phytopathology”, Russian Agricultural Academy”, st. Institute, own 5, Bolshie Vyazemy, Odintsovo distr., Moscow reg., 143050, Russia

^* Corresponding author: tanirtashir@mail.ru

Abstract

In order to increase the level of production of hydrolytic enzymes of natural producers and select highly productive microorganisms, a study was carried out on the effectiveness of various approaches to induced mutagenesis, as well as a comparative study of the ability of exoenzymes biosynthesis by original and mutant strains. New mutant strains of B. subtilis and T. harzianum with some morphological deviations were obtained using multi-stage non-directional mutagenesis in a fractional mode followed by selection on selective media. These bacteria were superior to the parent strains of B. subtilis MB085-148-72 and T. harzianum MF091-07-305 in the number of hydrolytic enzymes produced. The stability of the characteristics of the new producers was confirmed by three successive subcultures on nutrient media containing the appropriate substrates of hydrolytic enzymes. Considering the high level of production of hydrolases of new strains of microorganisms, they can be recommended as components of feed additives, biopreservatives for silage and haylage, as well as effective producers of enzymes.