| Issue |
BIO Web Conf.
Volume 117, 2024
International Conference on Life Sciences and Technology (ICoLiST 2023)
|
|
|---|---|---|
| Article Number | 01047 | |
| Number of page(s) | 9 | |
| DOI | https://doi.org/10.1051/bioconf/202411701047 | |
| Published online | 05 July 2024 | |
Decreased Recombination Frequency in Lead Contaminated Drosophila melanogaster
1 Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Malang, Jl. Raya Tlogomas 246 Malang 65144, Indonesia
2 Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jl. Semarang No. 5, Malang 65145, Indonesia
* Corresponding author : siti.zubaidah.fmipa@um.ac.id
Recombination frequency through testcross involving Drosophila melanogaster can be used as relevant data in assessing the effect of certain substances on organisms. This study aimed to analyze the effect of lead-contaminated culture media on the percentage of recombination events. D. melanogaster was selected as the model organism while crossing over was selected as the observed recombination event. Lead levels in each treatment were 0, 0.05, 0.075, and 0.1 grams. Crossing over data was collected by calculating the frequency of recombinant-type strains from testcross results involving wildtype strains and vestigial black double mutants. Successively, the recombinant frequencies in the 0-, 0.05-, 0.075-, and 0.1-gram lead groups were 32.40, 14.65, 0, and 0%. The results of the hypothesis test indicated that lead contamination had a significant effect on reducing the recombination frequency. Therefore, lead may negatively impact the molecular aspects that control recombination events. Because recombination is regulated by genes and involves various proteins, a decrease in recombination frequency indicates that lead has a negative impact on genes or proteins during gametogenesis.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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