| Issue |
BIO Web Conf.
Volume 121, 2024
Global Summit on Life Sciences and Bio-Innovation: From Agriculture to Biomedicine (GLSBIA 2024)
|
|
|---|---|---|
| Article Number | 01008 | |
| Number of page(s) | 4 | |
| Section | Bioengineering and Biotechnological Innovations | |
| DOI | https://doi.org/10.1051/bioconf/202412101008 | |
| Published online | 22 July 2024 | |
Obtaining aseptic in vitro culture of Hydrangea paniculata ‘Samarskya Lydia’
South-Ural Botanical Garden-Institute, Ufa Federal Research Centre, RAS, Ufa, Russia
* Corresponding author: zaripova.al@mail.ru
Techniques for obtaining sterile culture in vitro Hydrangea paniculata 'Samarskaya Lydia' have been developed. The highest number of viable (92%) explants with a minimum of infected and necrotic ones was obtained by sequential treatment of apical and axillary buds from spring regrowth shoots in 3% hydrogen peroxide solution for 3 minutes and 7% sodium hypochlorite solution for 4 minutes. The selected Anderson nutrient medium containing BAP at a concentration of 5.0 mg/l and IAA – 1.0 mg/l is the most preferable for regeneration of shoots with a propagation coefficient of 6.
© The Authors, published by EDP Sciences, 2024
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