| Issue |
BIO Web Conf.
Volume 127, 2024
The International Conference and Workshop on Biotechnology (ICW Biotech 2024)
|
|
|---|---|---|
| Article Number | 06001 | |
| Number of page(s) | 7 | |
| Section | Synthetic Biotechnology for Enhanching Benefit and Production of Bioprospecting | |
| DOI | https://doi.org/10.1051/bioconf/202412706001 | |
| Published online | 13 September 2024 | |
Increasing the production of insulin glargine in Pichia pastoris through medium modification
1 Research Center for Vaccine and Drug, Research Organization for Health, National Research and Innovation Agency (BRIN), South Tangerang, Banten, 15314, Indonesia
2 Research Center for Applied Botany, Research Organization for Life Sciences and Environment, National Research and Innovation Agency (BRIN), South Tangerang, Banten, 15314, Indonesia
3 EDP Sciences, Biology, Military MIPA, Defense University, Bogor, West Java, 16810, Indonesia
4 Biomedical Sciences, Medicine, Andalas University, Padang, West Sumatera, 25163, Indonesia
5 Research Center for Estate Crops, Research Organization for Agriculture and Food, National Research and Innovation Agency (BRIN), South Tangerang, Banten, 15314, Indonesia
* Corresponding author: dudihd@gmail.com
Insulin is the primary drug for managing diabetes mellitus, especially for individuals with type 1 diabetes mellitus. Producing insulin glargine, a long-acting insulin analogue, using Pichia pastoris is a notable advancement in biopharmaceutical manufacturing. This research aims to increase the yield of insulin glargine in Pichia pastoris. The effects of these mediums and vitamins on cell growth and insulin glargine expression levels were evaluated. The findings revealed that the addition of vitamins to the minimal medium (MM) and ½ basal salt medium (BSM) increased insulin glargine production. This study highlights the critical role of vitamins in maximizing the efficiency of insulin glargine production in Pichia pastoris. The addition of vitamins to MM and ½ BSM mediums enhances the production of insulin glargine.
© The Authors, published by EDP Sciences, 2024
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