| Issue |
BIO Web Conf.
Volume 139, 2024
International Scientific and Practical Conference “AGRONOMY – 2024” (AgriScience2024)
|
|
|---|---|---|
| Article Number | 05008 | |
| Number of page(s) | 5 | |
| Section | Plant Breeding, Seed Production and Biotechnology | |
| DOI | https://doi.org/10.1051/bioconf/202413905008 | |
| Published online | 15 November 2024 | |
Isolation and regeneration of cell suspension-derived Foeniculum vulgare protoplasts
Botany Department, Moscow Timiryazev Agricultural Academy, 127434, Moscow, Russian Federation
* Corresponding author: s.monakhos@rgau-msha.ru
Hybrid development incorporating lines with cytoplasmic male sterility (CMS) has become an increasingly effecting technique for utilizing heterosis in vegetable crops. Currently, somatic hybridization has become prevalent in the Apiaceae family, which includes carrots, celery, and leeks. Therefore, as a first step, a simple and efficient protocol for isolating and regenerating protoplasts is established for the commercial fennel hybrid “Dragon” as a source of CMS that will be transferred later into carrot via protoplast fusion. To this end, cell suspensions from Dragon were initiated as a source for protoplast isolation.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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