Association between gene polymorphism of TLR2 and serum level of it in atopic dermatitis in Al-Najaf Governorate, Iraq

¹ Basic Sciences Branch, College of Dentistry, University of Kufa, Najaf, Iraq
² Department of Biology, Faculty of Science, University of Kufa, Najaf, Iraq

^* Corresponding author: attaak.alghazali@uokufa.edu.iq

Abstract

The purpose of this study was to investigate the polymorphism of Toll-like receptor 2 (TLR2) in patients with atopic dermatitis using the T-ARMS-PCR technology. The levels of TLR2 in the serum of these patients were then determined using the enzyme-linked immunosorbent test (ELISA). For a period of 16 months, from 1/5/2022 to 1/8/2023, The 100 samples in this study were divided into two groups: the patients (70), whose clinical diagnosis of atopic dermatitis (AD) is confirmed by dermatologists, and the control groups (30), whose immunological and biochemical analyses confirmed their status. Patients and controls were arranged according to age and gender, with age groups further divided into five categories: 1-7, 7-16, 16-33, 33-45, and >45 years, about 70 out of 100 AD samples have TLR2 gene polymorphisms in CC, TT, and TC, and serum TLR2 levels were greater in patients than in controls (p-value=0.029*). In conclusion, atopi dermatitis may be caused by a gene polymorphism in TLR2 in (CC, TT, and TC). Additionally, immunological analysis revealed that AD patients had higher serum levels of TLR2 than did healthy individuals.