Silymarin attenuates Oxidative stress and Nephrotoxicity induced by 5-Fluorouracil in rats

Department. of Biology, Faculty of Science, University of Kufa, Najaf, Iraq

^* Corresponding author: ftr33opo@gmail.com

Abstract

The study aimed to appraise attenuator effect of the Silymarin (SLY) on Oxidative stress and Nephrotoxicity in 5- Fluorouracil treated rats. The study including 60 male rats weighting (180- 200 g) divided into 6 groups including group F (control) receive 0.5 ml normal saline and group A ( 5-Flu 5 mg /kg), group B (5-Flu 5mg /kg + SLY 3mg /kg), group C (SLY 3mg /kg) for 3o days, group D (SLY 3mg /kg for 15 days after that 5-Flu 5mg /kg for 15 days), group E (5-Flu 5mg /kg for 15 days after that SLY 3mg /kg for 15 days), after animals autopsied, the kidney samples obtained for histological examination and serum collected and stored at – 43 ℃ for biochemical tests. GSH test show no significant difference between group 5-Flu treated rats and control group, while groups treated SLY+5-Flu and SLY only showed a marked increase compared to control at (P≤ 0.05). Also the MDA test show groups treated with 5-Flu and 5-Flu +SLY significantly increase compared to control, while group treated with SLY show no significant difference compared to control at (P≤ 0.05). The histological examination show the acute tubular necrosis damage percentage elevated compared to control in group treated 5-Flu only, while groups treated with SLY+5-Flu and SLY showed improvement in damage percentage at (P≤ 0.05). In conclusion, the Silymarin may provide attenuator role against Oxidative stress and nephrotoxicity induced by 5-Flu drug in the groups that underwent different treatment periods.