| Issue |
BIO Web Conf.
Volume 139, 2024
International Scientific and Practical Conference “AGRONOMY – 2024” (AgriScience2024)
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|
|---|---|---|
| Article Number | 10004 | |
| Number of page(s) | 5 | |
| Section | Breeding, Selection and Genetics of Farm Animals | |
| DOI | https://doi.org/10.1051/bioconf/202413910004 | |
| Published online | 15 November 2024 | |
Interaction of ROX and ssGreen Fluorophores Affects Visualization of Gel Electrophoresis Results
FSBSI “Ural Federal Agrarian Scientific Research Centre, Ural Branch of Russian Academy of Sciences”, Sverdlovsk region, Ekaterinburg, Russia
* Corresponding author: bodrova-zaizeva@mail.ru
Fluorescent molecules have found wide application in biological and medical research: from flow cytometry and confocal microscopy to ELISA and PCR methods. Understanding the properties of fluorophores and their interactions is an integral part of developing new research methods in a wide range of scientific fields. In this paper, we present the results of the analysis of the interaction of carboxy-X-rhodamine (ROX) and the intercalating dye of the green spectrum for single-stranded DNA and RNA molecules – ssGreen. It was found that the presence of ssGreen in the agarose gel visually gives a faint enhance to the rhodamine signal compared to the gel without ssGreen. Moreover, the absence of green spectrum luminescence (quenching) was observed in the oligonucleotide with rhodamine in its composition, in contrast to the PCR primer without fluorophores in its composition. The proposed FRET phenomenon is not supported by the results of quantum mechanism calculations, which requires further study.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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