| Issue |
BIO Web Conf.
Volume 141, 2024
IX International Scientific Conference on Agricultural Science 2024 “Current State, Problems and Prospects for the Development of Agricultural Science” (AGRICULTURAL SCIENCE 2024)
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|---|---|---|
| Article Number | 01002 | |
| Number of page(s) | 8 | |
| Section | Plant Genetics and Breeding | |
| DOI | https://doi.org/10.1051/bioconf/202414101002 | |
| Published online | 21 November 2024 | |
Morphology, anatomy, ploidy level and genetic similarity investigation of the Prunus persica (L.) Batsch ‘Dostoyniy’ex situ and in vitro
1 Federal State Funded Institution of Science, The Labor Red Banner Order Nikita Botanical Gardens – National Scientific Center of the RAS, 298648 Yalta, Russia
2 Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, 295007 Simferopol, Russia
* Corresponding author: cellbiolnbs@yandex.ru
The article discusses the structure and genetic stability of the Prunus persica ‘Dostoyniy’ microshoots, culturing in vitro on the Gamborg and Eveleg (B5) nutrient medium compared with the shoots of the mother plants ex situ. Conventional methods were applied to study plant morphology, anatomy, ploidy level and genetic similarity. Statistical analysis was performed using the Past software. According to the results, common leaf morphology and its structure in vitro were preserved, but several quantitative and qualitative changes were revealed, except for rearrangements. The analysis of the ploidy level of the nuclei isolated from the leaf tissue cells of the microshoots in vitro revealed no changes. Similar results were obtained with ISSR-PCR and genetically material in vitro was stable. Short-term cultivation of Prunus persica ‘Dostoyniy microshoots on a B5 nutrient medium with optimal concentrations of 6- BAP promoted morphogenesis without significant deviations, and the absence of the ploidy level changes and genetic variations under in vitro conditions.
© The Authors, published by EDP Sciences, 2024
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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