| Issue |
BIO Web Conf.
Volume 141, 2024
IX International Scientific Conference on Agricultural Science 2024 “Current State, Problems and Prospects for the Development of Agricultural Science” (AGRICULTURAL SCIENCE 2024)
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|---|---|---|
| Article Number | 02012 | |
| Number of page(s) | 8 | |
| Section | Soil Biology and Biotechnology | |
| DOI | https://doi.org/10.1051/bioconf/202414102012 | |
| Published online | 21 November 2024 | |
Development of laboratory regulations elements for the pesticide production of based on the entomopathogenic strain Bacillus thuringiensis 0271
Research Institute of Agriculture of Crimea’, 295493, Kievskaya str.150, Simferopol, Russian Federation
* Corresponding author: nk_lib@mail.ru
The production of new entomocidal biologics based on Bacillus thuringiensis bacteria does not lose its relevance. In this regard, studies have been conducted on the individual elements development of the regulations for the production of biological insecticide. It has been shown that the liquid formulation obtained on nutrient media SG, PS and No. 9, without stabilizers, retains a spore titer of at least 1.2 billion/ml for 6 months. The culture of the strain B. thuringiensis 0271 obtained on nutrient media No. 7 and No. 9 is able to withstand heat treatment no higher than 70°C and no longer than 30 minutes. Simulation of fermentation conditions of strain B. thuringiensis 0271 made it possible to predict that the maximum spore titer can be achieved by maintaining a gas pressure of at least 0.7 air volume per medium volume per minute, increasing the temperature at the end of cultivation to 32°C, and a pH in the stationary phase of at least 8.0.
© The Authors, published by EDP Sciences, 2024
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