| Issue |
BIO Web Conf.
Volume 147, 2024
11th International Symposium of East Asia Fisheries and Technologist Association (EAFTA 2024)
|
|
|---|---|---|
| Article Number | 01028 | |
| Number of page(s) | 14 | |
| DOI | https://doi.org/10.1051/bioconf/202414701028 | |
| Published online | 10 January 2025 | |
Effect of Alkali Pretreatment on Enzymatic Hydrolysis of Gracilaria sp.
1 Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, IPB University, Jl Agatis Kampus IPB Dramaga, Bogor, West Java 16680, Indonesia.
2 Surfactant and Bioenergy Research Center (SBRC), IPB University, Bogor, Indonesia, 16810
3 Research Center for Chemistry-National Research and nnovation Agency Indonesia (BRIN), Setu, South Tangerang, Banten, 15314, Indonesia.
4 Research Center for Marine and Land Bioindustry-National Research and Innovation Agency (BRIN), Lombok, Indonesia
* Corresponding author: ujusadi@apps.ipb.ac.id
† Corresponding author: yann001@brin.go.id
Pretreatment of seaweed biomass for bioethanol production. This study aimed to determine the effect of biomass pretreatment with 5% NaOH alkali on enzymatic hydrolysis in the bioethanol production process using Gracilaria sp. as raw material. Pretreatment with 5% NaOH alkali was carried out with different time variations, namely 60, 90, and 120 minutes at a temperature of 50oC. After pretreatment, the enzymatic hydrolysis process was carried out using cellulase enzymes on a shaker incubator at a temperature of 50oC, 150 rpm for 72 hours, then the fermentation process for 72 hours using Saccharomices cerevisiae mold. The results showed that 5% NaOH alkali pretreatment could improve the quality of Gracilaria sp. biomass. The lignin content produced was 10.43 ± 0.15%.
© The Authors, published by EDP Sciences, 2024
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