Issue |
BIO Web Conf.
Volume 151, 2025
International Conference “Mountains: Biodiversity, Landscapes and Cultures” (MBLC-2024)
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Article Number | 01009 | |
Number of page(s) | 7 | |
Section | Agriculture and Ecology | |
DOI | https://doi.org/10.1051/bioconf/202515101009 | |
Published online | 21 January 2025 |
Genetic Diversity Analysis of Mulberry Silkworm (Bombyx Mori L.) Hybrids and Breeds in Azerbaijan Using RAPD Markers
1 Department of Natural Sciences, Western Caspian University, 17A, Ahmad Rajabli st., AZE1072, Baku Azerbaijan
2 Genetic Resources Institute of the Ministry of Science and Education, Baku, Azerbaijan
3 Department of Silkworms Breeding, Sheki Regional Scientific Center of ANAS, Baku, Azerbaijan
4 Department of Breeding and Nutrition of Agricultural Animals of the Azerbaijan State Agrarian University, Baku, Azerbaijan
* Corresponding author: ayaz.mammadov@wcu.edu.az
In order to study the genetic diversity of silkworms (Bombyx mori L.) breeding in Azerbaijan, the following breeds and hybrids were analyzed: SHZEM 4 x GE 143, Chingiz x Yagub, Yagub x Chingiz, GE 143, Yagub, SHZEM 4 belted, Chingiz, GE 143 x SHZEM 4 using RAPD molecular markers. For this purpose, silkworm larvae were taken for DNA extraction and nuclear genome DNA was isolated from various parts of the silkworm (silk gland, epidermis, etc.). Four RAPD primers (BGN 04, BGL 02, BGY 06, BGA 02) were used in the study, resulting in 200-1200 bp 34 dots representing fragments. The average number of amplicons for each primer was 6-11, and 82-100% of the amplicons were polymorphic. The study found that RAPD markers do not fully reflect the similarity between the local breed and hybrids.
Key words: silkworm / breeds / hybrids / homozygote / hereditary
© The Authors, published by EDP Sciences, 2025
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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