Issue |
BIO Web Conf.
Volume 151, 2025
International Conference “Mountains: Biodiversity, Landscapes and Cultures” (MBLC-2024)
|
|
---|---|---|
Article Number | 01010 | |
Number of page(s) | 10 | |
Section | Agriculture and Ecology | |
DOI | https://doi.org/10.1051/bioconf/202515101010 | |
Published online | 21 January 2025 |
Genetic Variation of Lentil Landraces Across Different Altitudes in Azerbaijan
1 Genetic Resources Institute of AR SEM, Molecular Genetics and Genomics department, Azadlig ave. 155, AZE 1106, Baku, Azerbaijan
2 Western Caspian University, Department of Natural Sciences, 17A, Ahmad Rajabli st., AZE1072, Baku, Azerbaijan
Lentil is mainly grown in rainfed systems and thus plays an important role in sustainable agriculture, especially in high mountain landscapes. The aim of this study was to assess the genetic diversity of 50 lentil accessions, including landraces from high mountain regions of Azerbaijan as well as wild lentil species, using SSR markers. DNA was extracted from leaves, amplified by PCR and separated by polyacrylamide gel electrophoresis. Statistical analysis included dendrogram construction and principal coordinate analysis (PCoA). A total of 26 alleles were identified, with an average of 8.6. Primers SSR 156 and SSR 323 showed transferability between all studied species/subspecies within the genus Lens. The genetic diversity index for each primer ranged from 0.704 to 0.776, with a mean value of 0.735. Accessions from higher altitudes such as Ismayilli and Nakhchivan contributed significantly to the observed genetic variation. The dendrogram revealed four clusters in which 71.4% of the accessions were distinguishable. Lens culinary subsp. odemensis formed a distinct subcluster due to its high genetic distance. PCoA analysis revealed a dispersed sample distribution, with the first five axes accounting for 69.9% of the total variation. The study revealed significant genetic diversity among lentil accessions in Azerbaijan, including highland regions. These results highlight the need for conservation and will support future breeding and sustainable agriculture.
© The Authors, published by EDP Sciences, 2025
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.