Sperm quality profile of Bali bull as local Indonesian cattle in liquid storage in different diluents with the addition of synthetic antioxidants

¹ Biology Department, Faculty of Mathematics and Natural Science, State University of Surabaya
² Artificial Insemination Centre, Singosari, Malang

^* Corresponding author: nurducha@unesa.ac.id

Abstract

Bali cattle are one of Indonesia’s local cattle, which require efforts to preserve genetic material from superior males by storing sperm at low temperatures so that it does not become extinct and can be implemented for artificial insemination. The simplest storage method is liquid storage, at 4-5°C. This study aimed to examine the quality profile of Bali bull sperm during storage at 4-5°C in CEP diluent with and without synthetic antioxidants (alpha-tocopherol, glutathione) and to compare it with the diluent media usually used by Indonesian Artificial Insemination centers for freezing bovine semen, namely tris aminomethane egg yolk. The research used 3 Balinese bull ejaculates, each replicated 3 times. Fresh semen was collected using an artificial vagina. The sperm quality profile included motility, viability, and membrane integrity a. Sperm motility was observed using the CASA IVOS 2 tool. Sperm viability was observed using the eosin nigrosine staining method under a microscope with 200 magnification. Membrane integrity was observed utilizing the HOST test (Hypo Osmotic Swelling Test). The results showed that the percentage of motility from the first day to the last day of storage was highest in CEP diluent with the addition of alpha-tocopherol and glutathione. The observations of membrane viability and integrity showed the highest percentage of CEP diluent with the addition of alpha-tocopherol from the first to the last day of storage. The research results show that Bali cattle sperm can be stored at low temperatures, especially in CEP diluent, with the addition of antioxidants.