| Issue |
BIO Web Conf.
Volume 176, 2025
International Conference on Blue Economy and Techno-Socio Environmental Innovation (INFLECTION 2024)
|
|
|---|---|---|
| Article Number | 02002 | |
| Number of page(s) | 11 | |
| Section | Marine Biotechnology | |
| DOI | https://doi.org/10.1051/bioconf/202517602002 | |
| Published online | 23 May 2025 | |
Screening and Characterization of Exopolysaccharide-Producing Endophytic Fungi
1 Departement of Aquatic Product Technology, Faculty of Fisheries and Marine Science, IPBUniversity, 16680 Bogor Indonesia
2 Division of Marine Biotechnology, Center for Coastal and Marine Resources Studies (PKSPL), International Research Institute for Maritime, Ocean and Fisheries (i-MAR), IPB University, 16127 Bogor, Indonesia
3 Surfactant and Bioenergy Research Center (SBRC), IPB University, 16143 Bogor, Indonesia
* Corresponding author: safrina_dyah@apps.ipb.ac.id
Marine endophytic fungi are microorganisms that inhabit marine plant tissues without harming their hosts. These fungi can produce exopolysaccharides (EPS), which have potential applications in food, pharmaceuticals, and biomaterials. This study aims to screen and characterize EPS-producing marine endophytic fungi. Ten fungi isolates from seaweed, seagrass, and mangroves were cultivated in Czapek-Dox medium, and EPS production was evaluated based on wet and dry biomass yields. Among the isolates, KTR49 produced the highest EPS yield (180±12.26 mg/L) and was further analyzed. FTIR spectroscopy confirmed the presence of hydroxyl (-OH), carbonyl (C=O), and glycosidic (C-O-C) bonds, indicating polysaccharide characteristics. Molecular identification using PCR and phylogenetic analysis classified KTR49 as closely related to Aspergillus flavus. Viscosity analysis showed moderate flow resistance (1.869 ± 0.054 mPa·s), and thermogravimetric analysis (TGA) indicated thermal stability up to 250°C.
Publisher note: The corresponding author’s email address has been added, on June 24, 2025.
© The Authors, published by EDP Sciences, 2025
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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