| Issue |
BIO Web Conf.
Volume 184, 2025
The 3rd International Seminar of the Indonesian Society for Biochemistry and Molecular Biology (ISISBMB 2025)
|
|
|---|---|---|
| Article Number | 03003 | |
| Number of page(s) | 7 | |
| Section | Marine Science and Biotechnology | |
| DOI | https://doi.org/10.1051/bioconf/202518403003 | |
| Published online | 12 August 2025 | |
DNA Barcoding and Phylogenetics of Freshwater Ornamental Fish Using Mitochondrial COI Gene
1 Aquatic Resources Management, Warmadewa University, Indonesia
2 Research Center for Fishery, Research Organization for Earth Sciences and Maritime, National Research and Innovation Agency, Indonesia.
3 Faculty of Agriculture, Udayana University
* Corresponding author: suryanip@rocketmail.com
The purpose of this study was to identify and conserve local fish that have the potential to be ornamental fish. The total sample was 16 fish. Methods employed were DNA isolation, polymerase chain reaction (PCR) and COI gene sequencing. The DNA sequences generated were compared with the sequences from NCBI using the MEGA 7 and BLASTn programs. The results showed that the size of the COI gene produced was 615 bp to 699 bp. As many as twelve species were identified to have the potential to be ornamental fish, namely Rasbora lateristriata, Barbodes binotatus, Xiphophorus helleri, Sicyopterus microcephalus, Puntigrus tetrazona, Moenkhausia santaefilomenae, Hyphessobrycon eques, Poecilia reticulata, Poecilia latipinna, Xiphophorus maculatus, Pethia conchonius, Poecilia orri. These results showed that the local Balinese fish tested had a 70-99% similarity. DNA barcoding and molecular identification can be used for the genetic conservation to prevent local fish populations from becoming extinct.
© The Authors, published by EDP Sciences, 2025
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.