| Issue |
BIO Web Conf.
Volume 189, 2025
11th International Conference on Sustainable Agriculture, Food, and Energy (SAFE 2025)
|
|
|---|---|---|
| Article Number | 01024 | |
| Number of page(s) | 10 | |
| Section | Sustainable Agriculture, Food, and Energy | |
| DOI | https://doi.org/10.1051/bioconf/202518901024 | |
| Published online | 09 October 2025 | |
Computer-Assisted Semen Analysis in Indonesian Buffalo: Correlations with Plasma Membrane Integrity, DNA Fragmentation, and Acrosome Integrity
1 Faculty of Animal Science, Hasanuddin University
2 Research Centre for Applied Zoology, National Research and Innovation Agency, Jl. Raya Bogor Km.46 Cibinong, Bogor 16914, Indonesia
3 Faculty of Agriculture, Sumatera Utara University
4 Regional Artificial Insemination Center, South Sulawesi, Indonesia
5 Department of Clinic, Reproduction, and Pathology, Faculty of Veterinary Medicine, IPB University, Jl. Agatis, Dramaga Campus, Bogor 16680, Indonesia
* Corresponding author: syah006@brin.go.id
This research investigated the association between structural quality and motility traits of cryopreserved semen in Silangit and Toraya buffalo using a computer-assisted semen analysis (CASA) system. Post-thaw examination revealed no statistical difference in plasma membrane integrity between the two breeds (Silangit: 62.5 ± 3.2%; Toraya: 63.1 ± 2.8%). In contrast, the level of DNA fragmentation was greater in Silangit bulls (18.4 ± 2.6%) compared with Toraya (12.7 ± 1.9%). Acrosome condition was generally well maintained in both groups (>95%). The CASA assessment further revealed that Toraya sperm exhibited superior values for average path velocity and lateral head displacement, while Silangit samples displayed higher straightness, linearity, and beat cross frequency. Overall, the study demonstrates that differences in membrane stability, DNA integrity, and acrosome status contribute to breed-specific motility profiles, emphasizing the relevance of CASA for designing semen preservation strategies in buffalo breeding.
© The Authors, published by EDP Sciences, 2025
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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