Cogon Grass Root Extract (Imperata cylindrica) Improves Endothelial Cells' Viability in Huvecs-Induced Angiotensin II

¹ Department of Medical, Faculty of Medicine, Universitas Islam Malang, Malang City, East Java, Indonesia
² Center for the Integration of Islamic Medicine, Bioinformatics, and Indonesian Traditional Medicine
³ Department of Pharmacology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
⁴ Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

¹ Corresponding author: This email address is being protected from spambots. You need JavaScript enabled to view it.

Abstract

Angiotensin II (Ang II) stimulates oxidative stress in endothelial cells, leading to cell dysfunction. The active substances of Imperata cylindrica roots (IC) extract inhibit oxidative stress. This study aimed to evaluate whether IC extract treatment increases Ang II-induced endothelial cell viability (ECV). The in vitro model on human umbilical vein endothelial cells culture (HUVECs) treated with 0.1 μM Ang II. There were five groups: control (CTL), Ang II, and three groups each given Ang II and 5, 10, and 15 μg/ml IC extract, respectively. Cell Counting Kit-8 (CCK-8) was used to assess ECV at 0, 24, and 48 hours, respectively. The data were analysed using one-way ANOVA. A p-value less than 0.05 was considered significant. ECV percentage at 24-hour observation in the CTL, Ang II, 5, 10, 15 μg/ml IC extract groups was 100±1.17; 94.91±0.75; 96.16±0.76; 95.87±1.75, and 95.65±0.97%, respectively. At 24-hour observation was 100±0.89; 78.71±0.38; 98.84±0.99; 111.99±0.99, and 101.77±1.76%, respectively. Further, at 48-hour observation was 100±0.83; 59.23±2.21; 107.83±3.94; 129.39±0.62, and 136.51±2.99%, respectively. Ang II reduces ECV compared to the control group at time-dependent observations (p<0.000). However, IC extract significantly improved ECV at all doses compared to the Ang II group (p < 0.000). IC extract increases Ang II-induced ECV.