Development of In vitro Maturation and In vitro Growth Culture Media Using Goat Colostrum

Faculty of Animal Sciences, Universitas Brawijaya, Malang 65145, Indonesia

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Abstract

This research aims to develop a more optimal in vitro maturation (IVM) oocyte and in vitro growth (IVG) follicle culture medium through the utilization of goat colostrum as an additive to the standard IVM and IVG culture medium. This research was conducted at the Bioscience Laboratory of Brawijaya University, Malang, using goat ovary samples obtained from the Gadang Slaughterhouse (RPH), Malang Regency. This research was carried out in two stages, namely IVG and IVM, with the data obtained subsequently analyzed using ANOVA followed by LSD for significance testing. IVG was performed on goat follicles with an initial size of 2-3 mm, followed by culture for 6 days using media treated with the addition of goat colostrum from P0 to P4, respectively, 0%, 2.5%, 5%, 7.5%, and 10%. The IVG culture results obtained were observed in terms of follicle size increase, oocyte cell size, and cumulus cell expansion of the oocytes within the follicles. The results obtained were that IVG had no significant effect on follicle size growth (P>0.05), but showed a significant effect on cumulus cell expansion (P<0.05) with the highest value in P4=2.5 and the lowest in P1=1.545. Meanwhile, for oocyte cell size, it also showed a significant effect (P<0.05) with the largest size being P4=195.0 μm and the smallest being P0=168.7 μm. For the next stage, IVM was performed on oocytes for 24 hours with media receiving the same treatments, namely P0 to P4 with goat colostrum concentrations respectively 0%, 2.5%, 5%, 7.5%, and 10%; the IVM culture results were observed in terms of oocyte cell size increase and cumulus cell expansion quality. The IVM oocyte results obtained showed a significant difference in oocyte size increase (P<0.05), with the largest growth in P1 at 34.3 μm and the smallest in P0 at 12.0 μm. Meanwhile, for cumulus cell expansion, a significant difference was also obtained (P<0.05) with the best average in P4=3.20 and the smallest in P0=1.66. Overall, goat colostrum has potential as an additive to culture media, particularly in improving cumulus cell expansion in IVG and IVM.