Open Access
Table 1
Influence of culture medium composition and cultivar on the induction of calluso- and morphogenesis in Lavandula angustifolia in vitro
| Cultivar | Culture medium for obtaining primary callus | The frequency of induction, % of the number of explants | Nutrient medium in the first passage | Frequency of morphogene sis in the first passage, % | ||
|---|---|---|---|---|---|---|
| medium | hormonal supplements in a MS medium, (mg/l) | callusog enesis | indirect morphoge nesis | |||
| Vdala | MS160 | NAA (1.0)+BAP(0.5) | 97.6±8.6 | 0 | MS549 | 0 |
| MS554 | 0 | |||||
| MS544 | Kin (1.0) | 56.5±7.0 | 0 | MS552 | 0 | |
| MS546 | Kin (1.0)+ GA3 (0.5) | 63.4±5.4 | 0 | MS546 | 0 | |
| MS552 | 0 | |||||
| MS549 | BAP (1.0) | 62.9±72 | 0 | MS549 | 5.7±0.4 | |
| MS550 | BAP (2.0) | 66.7±7.0 | 0 | MS550 | 32.5±3.7 | |
| MS551 | BAP (1.0)+ GA3 (0.5) | 58.8±65 | 0 | MS552 | 20.0±1.9 | |
| MS550 | 0 | |||||
| MS552 | Kin (1.0)+ BAP (1.0) | 65.5±52 | 2.5±0.9 | MS552 | 43.2±45 | |
| MS554 | TDZ (1.0) | 77.7±72 | 0 | MS554 | 0 | |
| MS556 | TDZ (1.0)+ GA3 (0.5) | 73.5±7.1 | 5.7±1.2 | MS556 | 9.7±1.1 | |
| Stepnaya | MS160 | NAA (1.0)+ BAP(0.5) | 100.0 | 0 | MS549 | 0 |
| MS554 | 0 | |||||
| MS544 | Kin (1.0) | 48.5±52 | 0 | MS544 | 0 | |
| MS546 | Kin (1.0)+ GA3 (0.5) | 59.7±52 | 2.2±1.0 | MS546 | 18.5±2.0 | |
| MS549 | BAP (1.0) | 68.8±5.7 | 1.5±0.8 | MS549 | 20.0±2.1 | |
| MS550 | 0 | |||||
| MS550 | BAP (2.0) | 67.6±8.4 | 11.6±1.7 | MS550 | 18.5±2.0 | |
| MS551 | BAP (1.0)+ GA3 (0.5) | 71.5±68 | 10.8±1.2 | MS551 | 15.6±1.3 | |
| MS552 | Kin (1.0)+ BAP (1.0) | 72.3±7.8 | 2.2±1.0 | MS552 | 39.5±3.4 | |
| MS554 | TDZ (1.0) | 56.7±52 | 3.5±0.6 | MS554 | 9.7±1.3 | |
| MS556 | 6.8±0.9 | |||||
| MS556 | TDZ (1.0)+ GA3 (0.5) | 65.0±52 | 5.8±0.8 | MS556 | 28.8±2.1 | |
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