Experimental field design. Tempranillo plants defence response will be evaluated (in S1 and S2 samplings), while plants nutritional status (in S3 and S4). The antimicrobial efficiency of treatments will be followed in vivo through all the experiment (S1-S5) by measuring disease incidence/severity, as well in vitro (in S3 and S4). Moreover, the shifts on the native microbiota will be studied (in S3, S4 and S5). Grapevine yield and grape quality will be evaluated (in S5), and must and wine composition will be assessed (in S6).