PCR screening of feed products for the detection of genetically modified soybean

Open Access

Issue		BIO Web Conf. Volume 170, 2025 71^st International Scientific Conference “FOOD SCIENCE, ENGINEERING AND TECHNOLOGY – 2024”


Article Number		02007
Number of page(s)		6
Section		Food Chemistry, Microbiology and Biotechnology
DOI		https://doi.org/10.1051/bioconf/202517002007
Published online		01 April 2025

R. Batista, M.M. Oliveira, Facts and fiction of genetically engineered food. Trends Biotechnol. 27 (5), 277-286 (2009). https://doi.org/10.1016/j.tibtech.2009.01.005 [Google Scholar]
European Commission. Regulation (EC) No 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed. Official Journal of the European Union 2003a; L268:1-23 [Google Scholar]
GM Crop Events List, 2024. Available from: https://www.isaaa.org/gmapprovaldatabase/eventslist/default.asp [Google Scholar]
A. S. Bawa, K. R. Anilakumar, Genetically modified foods: safety, risks and public concerns-a review. J Food Sci Technol. 50 (6), 035-46 (2013). https://doi.org/10.1007/s13197-012-0899-1 [Google Scholar]
E. Rosculete, E. Bonciu, C. A. Rosculete, E. Teleanu, Detection and Quantification of Genetically Modified Soybean in Some Food and Feed Products. A Case Study on Products Available on Romanian Market. Sustainability. 10, 1325 (2018). https://doi.org/10.3390/su10051325 [Google Scholar]
P. Stefanova, G. Angelova, T. Georgieva. V. Gotcheva, A. Angelov, A novel Multiplex PCR Method for Simultaneous Detection of Genetically Modified Soybean Events. IJCMAS. 4 (4), 256-268 (2015). [Google Scholar]
A. Holst-Jensen, Testing for genetically modified organisms (GMOs): Past, present and future perspectives. Biotechnol. Adv. 27, 1071-1082 (2009). https://doi.org/.10.1016/j.biotechadv.2009.05.025 [Google Scholar]
C. Peano, R. Bordoni, M. Gulli, A. Mezzelani, M. C. Samson, G. De Bellis, N. Marmiroli, Multiplex polymerase chain reaction and ligation detection reaction/universal assay technology for the traceability of genetically modified organisms in foods. Anal. Biochem. 346, 90-100 (2005). https://doi.org/10.1016/j.ab.2005.08.004 [Google Scholar]
E. Barbau-Piednoir, A. Lievens, E. Mbongolo-Mbella, N. Roosens, M. Sneyers, A. Leunda-Casi, and M. Van den Bulcke, SYBR®Green qPCR screening methods for the presence of “35S promoter” and “NOS terminator” elements in food and feed products. Eur. Food Res. Technol. 230, 383-393 (2010). https://doi.org/10.1007/s00217-009-1170-5 [Google Scholar]
C. Yoke-Kqueen, C. Yee-Tyan, K. Siew-Ping, R. Son, Development of multiplex-PCR for Genetically Modified Organism (GMO) detection targeting EPSPS and Cry1Ab genes in soy and maize samples. Int. Food Res. J. 18 (2) (2011) [Google Scholar]
A. Holst-Jensen, Y. Bertheau, M. Loose, L. Grohmann, S. Hamels, L. Hougs, D. Morisset, S. Pecora-ro, M. Pla, M. Van den Bulcke, D. Wulff, Detecting un-authorized genetically modified organisms (GMOs) and derived materials. Biotechnol. Adv. 30 (6), 1318-1335 (2012). https://doi.org/10.1016/j.biotechadv.2012.01.024 [Google Scholar]
I. Scholtens, E. Laurensse, B. Molenaar, S. Zaaijer, H. Gaballo, P. Boleij, A. Bak, E. Kok, Practical experiences with an extended screening strategy for genetically modified organisms (GMOs) in real-life samples. J. Agric. Food Chem. 61 (38), 9097-9109 (2013). https://doi.org/10.1021/jf4018146 [CrossRef] [PubMed] [Google Scholar]
S. S. Al Mazrooei, R. Dalal, D. Alreshidi, PCR- screening of genetically modified organisms in food and feed products sold in Kuwait's market. KJS. 51 (1), 100074 (2024). https://doi.org/10.1016/j.kjs.2023.06.004 [Google Scholar]
P. Safaei, S. Rezaie, M. Alimohammadi, S. A. K. Afshari, M. Mehdizadeh, E. M. Aghaee, Qualitative PCR-based detection of genetically modified soy and maize products in Iran. Int. J. Food Prop. 23 (1), 459–469 (2020). https://doi.org/10.1080/10942912.2020.1734613 [Google Scholar]
P. Stefanova, M. Taseva, T. Georgieva, V. Gotcheva, A. Angelov, A modified CTAB method for DNA extraction from soybean and meat products. Biotechnol. Biotechnol. Equip. 27(3), 3803–3810 (2013). https://doi.org/10.5504/bbeq.2013.0026 [CrossRef] [Google Scholar]
R. Meyer, F. Chardonnens, P. Hübner, J. Lüthy, Polymerase chain reaction (PCR) in the quality and safety assurance of food: Detection of soya in processed meat products. Z Lebensm Unters Forch. 203, 339-44 (1996). https://doi.org/10.1007/BF01231072 [Google Scholar]
M. Lipp, P. Brodmann, K. Pietsch, J. Pauwels, E. Anklam, IUPAC collaborative trial study of a method to detect genetically modified soybeans and maize in dried powder. J. AOAC Int. 82 (4), 923-928 (1999). https://doi.org/10.1007/BF01231072 [Google Scholar]
D. James, A.-M. Schmidt, E. Wall, M. Green, S. Masri, Reliable detection and identification of genetically modified maize, soybean and canola by multiplex PCR analysis. J. Agric. Food Chem. 51, 5839-5834 (2003). https://doi.org/10.1021/jf0341159 [Google Scholar]
A. Pirondini, U. Bonas, E. Maestri, G. Visioli, M. Marmiroli, N. Marmiroli, Yield and amplificability of different DNA extraction procedures for traceability in the dairy food chain. Food Control. 21 (4), 663-668 (2010). https://doi.org/10.1016/j.foodcont.2009.10.004 [Google Scholar]
S. R. Murray, R. C. Butler, G. M. Timmerman- Vaughan, Quantitative real-time PCR assays to detect DNA degradation in soy-based food products. J Sci Food Agric. 89 (7), 1137–1144 (2009). https://doi.org/10.1002/jsfa.3563 [Google Scholar]
N. Gryson, Effect of food processing on plant DNA degradation and PCR-based GMO analysis: a review. Anal. Bioanal. Chem. 396, 2003-2022. (2010). https://doi.org/10.1007/s00216-009-3343-2 [Google Scholar]
J. Greilhuber, R. Obermayer, Genome size and maturity group in Glycine max (soybean). Heredity. 78, 547-551 (1997). https://doi.org/10.1038/hdy.1997.85 [Google Scholar]
F.C. Marcelino, M. F. M. Guimaraes, E. G. De-Barros, Detection and quantification of Roundup Ready® soybean residues in sausage samples by conventional and real-time PCR. Ciência e Tecnologia de Alimentos. 28, 38-45 (2008). https://doi.org/10.1590/S0101-20612008000500007 [Google Scholar]
M. E. Rott, T. S. Lawrence, E. M. Wall, M. J. Green, Detection and quantification of Roundup Ready soy in foods byconventional and real-time polymerase chain reaction. J. Agric. Food Chem. 52 (16), 5223-5232 (2004). https://doi.org/10.1021/jf030803g [CrossRef] [PubMed] [Google Scholar]
P. Cardarelli, M. R. Branquinho, R. T. B Ferreira, F. P. da Cruz, A. L. Gemal, Detection of GMO in food products in Brazil: the INCQS experience. Food Control. 16, 859-866 (2005). https://doi.org/10.1016/j.foodcont.2004.07.010 [Google Scholar]
K. Taski-Ajdukovic, Z. Nikolic, M. Vujakovic, M. Milosevic, M. Ignjatov, D. Petrovic, Detection of genetically modified organisms in processed meat products on the Serbian food market. Meat Sci. 81 (1), 230-232 (2009). https://doi.org/10.1016/j.meatsci.2008.07.024 [CrossRef] [Google Scholar]
R. Greiner, U. Konietzny, Presence of genetically modified maize and soy in food products sold commercially in Brazil from 2000 to 2005. Food Control, 19, 499-505 (2008). https://doi.org/10.1080/10942912.2020.1734613 [Google Scholar]
M. Singh, R. K. Bhoge, G. Randhawa, Crop-specific GMO matrix multiplex PCR: A cost-efficient screening strategy for genetically modified maize and cotton events approved globally. Food Control. 70, 271-280 (2016). https://doi.org/10.1016/j.foodcont.2016.05.032 [Google Scholar]

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