Gastropod and Bivalvia Diversity Using Environmental DNA (E-DNA) Techniques in The Waters of Gili Genting and Gili Labak Islands, Sumenep, Madura

Open Access

Issue		BIO Web Conf. Volume 183, 2025 International Conference on Life Sciences and Technology (ICoLiST 2024)


Article Number		01009
Number of page(s)		10
DOI		https://doi.org/10.1051/bioconf/202518301009
Published online		09 July 2025

I. Insafitri, E.N.N. Asih, W.A. Nugraha, Marine tourism in Gili Labak Island: Willingness to pay method as an effort to preserve coral reef in Gili Labak Island, Madura, Indonesia. Aquaculture, Aquarium, Conservation & Legislation, 13(6), 3789-3797 (2020). [Google Scholar]
Y.T.A. Rukmana, T. Purnomo, T.Keanekaragaman dan Kelimpahan Gastropoda di Pantai Barung Toraja Sumenep, Madura. Lentera Bio Berk. Ilm. Biol., 8, 150-155 (2019). [Google Scholar]
T. Wiharti, N.R. Hanik, Identification of Types of Fish Captured by Fishermen at TPI Wuryantoro Wonogiri that are Consumed by the Community. Jurnal Biologi Tropis, 22(4), 1177-1187 (2022). https://doi.org/10.29303/jbt.v22i4.4137. [Google Scholar]
M.D. Hasbunallah, U.A. Pratama, T.A. Firmansyah, E.P. Fajar, A. Saputra, Feliyanti. Analisis Jenis Kerang Bivalvia di Pulau Setan Kawasan Mandeh Sumatera Barat. Prosiding SEMNAS BIO. 126-136 (2022). [Google Scholar]
M.G. Na'u, G. Pakaenoni, W.A. Blegur, Keanekaragaman dan Kelimpahan Bivalvia di Pantai Wini Kecamatan Insana Utara Kabupaten Timor Tengah Utara. Journal Science of Biodiversity. 3(1), 22-32 (2022). [Google Scholar]
V.N. Djalil, A. Farajallah, Y. Wardiatno, Aplikasi Teknik Environmental DNA (eDNA) untuk Deteksi Spesies Cheraxquadricarinatus (VON MARTENS 1868) menggunakan Sampel Air. Jurnal Biologi Tropis, 18(2), 134-140 (2018). https://doi.org/10.29303/jbt.v18i2.878. [Google Scholar]
M. Leray, N. Knowlton, DNA Barcoding and Metabarcoding of Standarized Samples Reveal Patterns of Marine Benthic Diversity. Proceedings of the National Academy of Sciences. 112(7), 2076-2081 (2015). [Google Scholar]
H. Madduppa, N.K.D. Cahyani, A.W. Anggoro, B. Subhan, E. Jefri, L.M.I. Sani, D. Arafat, N. Akbar, D.G. Bengen, eDNA metabarcoding illuminates species diversity and composition of three phyla (chordata, mollusca and echinodermata) across Indonesian coral reefs. Biodiversity and Conservation, 30(11), pp.3087-3114 (2021). [CrossRef] [Google Scholar]
T. Fukuzawa, H. Shirakura, N. Nishizawa, H. Bafata, Y. Kameda, H. Doi, Environmental DNA Extraction Method from Water for a High and Consistent DNA Yield. Environmental DNA. 5(4), 627-633 (2022). [Google Scholar]
E. Jue, D. Witters, R.F. Ismagilov, Two-Phase Wash to Solve the Obiquitous Contaminant-Carryover Problem in Commercial Nucleic-acid Extraction Kits. Scientific Reports. 10, 1-16 (2020). [Google Scholar]
Sunarno., F. Muna, N. Fitri, A. Malik, A. Karuniawati, A. Soebandrio, Metode Cepat Ekstraksi DNA Corynebacterium diphteriae untuk Pemeriksaan PCR. Buletin Penelitian Kesehatan. 42(2), 85-92 (2014). [Google Scholar]
P.A. Riupassa, Perancangan Primer Oligonukleotida untuk Polimerisasi in Vitro Gen Sukrosa Sintase. Biosfera. 26(3), 131-138 (2009). [Google Scholar]
M. Leray, J.Y. Yang, C.P. Meyer, S.C. Mills, N. Agudelo, V. Ranwez, J.T. Boehm, R.J. Machida, A New Versatile Primer Set Targeting a Short Fragment of the Mitochondrial COI Region for Metabarcoding Metazoan Diversity: Application for Characterizing Coral Reef Fish Gut Contents. Frontiers in Zoology. 10(34), 1-14 (2013). [Google Scholar]
R. Setyawati, S. Zubaidah, Optimasi Konsentrasi Primer dan Suhu Annealing dalam Mendeteksi Gen Leptin pada Sapi Peranakan Ongole (PO) Menggunakan Polymerase Chain Reaction (PCR). Indonesian Journal of Laboratory, 4(1), 36 (2021). https://doi.org/10.22146/ijl.v4i1.65550. [Google Scholar]
M. Jannah, Optimalisasi Kondisi PCR untuk Amplifikasi Sekuen Gen HBB. Jurnal Pendidikan Biologi. 12(1), 36-42 (2023). [Google Scholar]
K. Ratnayani, S.C. Yowani, L.S. Syane, Amplifikasi Fragmen 0,4 KB Daerah D-Loop DNA Mitokondria Lima Individu Suku Bali Tanpa Hubungan Kekerabatan dengan Metode Polymerase Chain Reaction (PCR). Jurnal Kimia. 3(1), 14-20 (2009). [Google Scholar]
H. Latuconsina, M.N. Nessa, R.A. Rappe, Komposisi Spesies dan Struktur Komunitas Ikan Padang Lamun di Perairan Tanjung Tiram - Teluk Ambon Dalam. Jurnal Ilmu dan Teknologi Kelautan Tropis. 4(year>1), 35-46 (2012). [Google Scholar]
E. Bolyen, J.R. Rideout, M.R. Dillon, N.A. Bokulich, C.C. Abnet, G.A. Al-Ghalith, H. Alexander, E.J. Alm, M. Arumugam, F. Asnicar, Y. Bai, Y., Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2. Nature biotechnology, 37(8), 852-857 (2019). [Google Scholar]
B.J. Callahan, P.J. McMurdie, S.P. Holmes, S.P., Exact sequence variants should replace operational taxonomic units in marker-gene data analysis. The ISME journal, 11(12), 2639-2643 (2017). [Google Scholar]
H.D. Ismail, H.D., Bioinformatics: a practical guide to NCBI databases and sequence alignments. Chapman and Hall/CRC (2022). [CrossRef] [Google Scholar]
S.F. Altschul, W. Gish, W. Miller, E.W. Myers, D.J. Lipman, D.J., Basic local alignment search tool. Journal of molecular biology, 215(3), 403-410 (1990). [Google Scholar]
P.J. McMurdie, S. Holmes, Waste not, want not: why rarefying microbiome data is inadmissible. PLoS computational biology, 10(4), p.e1003531 (2014). [Google Scholar]
P.D.N. Hebert, A. Cywinska, S.L. Ball, J.R. DeWaard, Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270(1512), 313-321 (2003). https://doi.org/10.1098/rspb.2002.221 [Google Scholar]
S.H. Lee, J.S. Lee, Three unrecorded marine gastropods from Korea. Korean J. Malacol, 36(2), 59-62 (2020). [Google Scholar]
L.M. Prayogo, Mangrove Vegetation Mapping Using Sentinel-2A Imagery Based on Google Earth Engine Cloud Computing Platform. International Journal of Science, Engineering, an Information Technology. 6(1), 249-255 (2021). [Google Scholar]
N. Yahya, I. Idris, N.S. Rosli, Z. Bachok, Mangrove-associated bivalves in Southeast Asia: A review. Regional Studies in Marine Science, 38, p.101382 (2020). [Google Scholar]
F. Pakaya, A.H. Olii, C. Panigoro, Keanekaragaman dan Kelimpahan Bivalvia pada Ekosistem Mangrove di Desa Menanggu, Kabupaten Boalemo. Jurnal Ilmiah Perikanan dan Kelautan. 9(3), 53 - 57 (2021). [Google Scholar]
Z. Ahmad, M. Junaidi, B.H. Astriana, Pengaruh Kepadatan Spat Kerang Mutiara (Pinctada maxima) dengan Metode Longline terhadap Pertumbuhan dan Kelangsungan Hidup. Jurnal Biologi Tropis. 19(2), 221 - 228 (2019). [Google Scholar]
A. Erika, I. Akhrianti, M. Hudatwi, Identifikasi Jenis Bivalvia pada Ekosistem Mangrove di Sekitar Perairan Kota Pangkalpinang. Journal of Marine Research. 11(4), 696-705 (2022). [Google Scholar]

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.