Prioritization Strategy and Engineering Optimization for Paclitaxel Biosynthesis: From Pathway Analysis to Synthetic Biology

Open Access

Issue		BIO Web Conf. Volume 237, 2026 2026 8^th International Conference on Biotechnology and Biomedicine (ICBB 2026)


Article Number		02008
Number of page(s)		10
Section		Pharmacology, Natural Products and Drug Delivery
DOI		https://doi.org/10.1051/bioconf/202623702008
Published online		10 June 2026

M.B. Martin, S.V. Angeloni, P. Garcia-Morales, P.F. Sholler, M.D. Castro-Galache, J.A. Ferragut, and M. Saceda, Regulation of estrogen receptor-alpha expression in MCF-7 cells by taxol. J. Endocrinol 180, 487–496 (2004) [Google Scholar]
Y. Zhang, L. Wiese, H. Fang, S. Alseekh, L. Perez de Souza, F. Scossa, J. Molloy, M. Christmann, and A.R. Fernie,Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis. Mol. Plant 16, 1951–1961 (2023) [Google Scholar]
T. Wang, L. Li, W. Zhuang, F. Zhang, X. Shu, N. Wang, and Z. Wang, Recent Research Progress in Taxol Biosynthetic Pathway and Acylation Reactions Mediated by Taxus Acyltransferases. Molecules 26, 2855 (2021) [Google Scholar]
P. Zeppini and J.C.J.M. van den Bergh, Competing recombinant technologies for environmental innovation: extending Arthur’s model of lock-in. Ind. Innov. 18, 317–334 (2011) [Google Scholar]
S. Jennewein, R. Croteau, Taxol: biosynthesis, molecular genetics, and biotechnological applications. Appl. Microbiol. Biotechnol. 57, 13–19 (2001) [Google Scholar]
J.Y. Yin, M. Lai, X.Y. Yu, D.D. Su, X.Y. Xiong, and Y.L. Li, Comprehensive strategies for paclitaxel production: insights from plant cell culture, endophytic microorganisms, and synthetic biology. Hort. Res. 12, uhae346 (2024) [Google Scholar]
A.L. Demain and P. Vaishnav, Natural products for cancer chemotherapy. Microbiol. Biotechnol. 4, 687–699 (2011) [Google Scholar]
Y.J. Hu, C.C. Gu, X.F. Wang, L. Min, and C.C. Li, Asymmetric Total Synthesis of Taxol. J. Am. Chem. Soc. 143, 17862–17870 (2021) [CrossRef] [PubMed] [Google Scholar]
P. Schoch, Y. Krivolapova, F. Schneider, L. Pan, and T. Gaich, Gram‐Scale Access to (3, 11)‐ Cyclotaxanes—Synthesis of 1‐Hydroxytaxuspine C. Angew. Chem. Int. Ed. 64, e202506245 (2025) [Google Scholar]
F.S. Sun, S.Y. Dang, D.R. Zheng, H. Zhang, Y. Wang, Y.H. Li, and H. Wu, Advances in paclitaxel biosynthesis and transcriptional regulation mechanisms. Shengwu Gongcheng Xuebao 40, 1380–1405 (2024) [Google Scholar]
E. Perez-Matas, D. Hidalgo-Martinez, A. Escrich, M.A. Alcalde, E. Moyano, M. Bonfill, and J. Palazon, Genetic approaches in improving biotechnological production of taxanes: An update. Front. Plant Sci. 14, 1100228 (2023) [Google Scholar]
A.S.A. El-Sayed, M.T. El-Sayed, A. Rady, N. Zein, G. Enan, A. Shindia, S. El-Hefnawy, M. Sitohy, and B. Sitohy, Exploiting the Biosynthetic Potency of Taxol from Fungal Endophytes of Conifers Plants; Genome Mining and Metabolic Manipulation. Molecules 25, 3000 (2020) [Google Scholar]
X. Cao, L. Xu, J. Wang, M. Dong, C. Xu, G. Kai, W. Wan, and J. Jiang, Endophytic fungus Pseudodidymocyrtis lobariellae KL27 promotes taxol biosynthesis and accumulation in Taxus chinensis. BMC Plant Biol. 22, 12 (2022) [Google Scholar]
D. Noack, M. Roth, R. Geuther, G. Müller, K. Undisz, C. Hoffmeier, and S. Gáspár, Maintenance and genetic stability of vector plasmids pBR322 and pBR325 in Escherichia coli K12 strains grown in a chemostat. Mol. Gen. Genet. 184, 121–124 (1981) [Google Scholar]
B. Jiang, L. Gao, H. Wang, Y. Sun, X. Zhang, H. Ke, S. Liu, P. Ma, Q. Liao, Y. Wang, H. Wang, Y. Liu, R. Du, T. Rogge, W. Li, Y. Shang, K.N. Houk, X. Xiong, D. Xie, S. Huang, X. Lei, and J. Yan, Characterization and heterologous reconstitution of Taxus biosynthetic enzymes leading to baccatin III. Science 383, 622–629 (2024) [Google Scholar]
M.E. Bergman, B. Davis, M.A. Phillips, Medically useful plant terpenoids: biosynthesis, occurrence, and mechanism of action. Molecules 24, 3961 (2019) [Google Scholar]
J. Hefner, S.M. Rubenstein, R.E. Ketchum, D.M. Gibson, R.M. Williams, and R. Croteau, Cytochrome P450-catalyzed hydroxylation of taxa-4(5), 11(12)-diene to taxa-4(20), 11(12)-dien-5 alpha-ol: The first oxygenation step in taxol biosynthesis. Chem. Biol. 3, 479–489 (1996) [Google Scholar]
S. He, I.I. Abdallah, R. van Merkerk, and W.J. Quax, Insights into taxadiene synthase catalysis and promiscuity facilitated by mutability landscape and molecular dynamics. Planta 259, 87 (2024) [Google Scholar]
C. Yang, Y. Wang, Z. Su, L. Xiong, P. Wang, W. Lei, X. Yan, D. Ma, G. Zhao, and Z. Zhou, Biosynthesis of the highly oxygenated tetracyclic core skeleton of Taxol. Nat. Commun. 15, 2339 (2024) [Google Scholar]
Y.M. Wu, Q.G. Liao, Y. Shang, Z.P. Gong, and J.B. Gou. Recent progress of paclitaxel biosynthesis aided by multi-omics. Plant Science Journal 40, 853–866 (2022) [Google Scholar]
S. Li, Y. Li, and C.D. Smolke, Strategies for microbial synthesis of high-value phytochemicals. Nat. Chem. 10, 395–404 (2018) [Google Scholar]
C.J. McClune, J.C. Liu, C. Wick, R. De La Peña, B.M. Lange, Discovery of FoTO1 and Taxol genes enables biosynthesis of baccatin III. Nature 643, 582–592 (2025) [Google Scholar]
J.C. Liu, R. De La Peña, C. Tocol, and E.S. Sattely, Reconstitution of early paclitaxel biosynthetic network. Nat. Commun. 15, 1419 (2024) [Google Scholar]
J. Zhong, Y. Wang, Z. Chen, Y. Yalikun, L. He, T. Liu, and G. Ma, Engineering cyanobacteria as a new platform for producing taxol precursors directly from carbon dioxide. Biotechnol. Biofuels Bioprod. 17, 99 (2024) [Google Scholar]
P. Chen, X. Liu, C. Gu, P. Zhong, N. Song, M. Li, Z. Dai, X. Fang, Z. Liu, J. Zhang, R. Tang, S. Fan, and X. Lin, A plant-derived natural photosynthetic system for improving cell anabolism. Nature 612, 546–554 (2022) [Google Scholar]
S. Jennewein, C.D. Rithner, R.M. Williams, and R. Croteau, Taxoid metabolism: taxoid 14β-hydroxylase is a cytochrome P450-dependent monooxygenase. Arch. Biochem. Biophys. 413, 262–270 (2003) [Google Scholar]
H. Rodríguez, N. Berghoff, J.S. Suchodolski, and J.M. Steiner, Kinetic analysis of 5 sugar probes in dog serum after orogastric administration. Can. J. Vet. Res. 73, 217–223 (2009) [Google Scholar]
K. Peleckis, Determining the level of market concentration in the construction sector—case of application of the HHI index. Sustainability 14, 779 (2022) [Google Scholar]
T. Bol, M. de Vaan, and A. van de Rijt, The Matthew effect in science funding. Proc. Natl. Acad. Sci. U.S.A. 115, 4887–4890 (2018) [Google Scholar]
M.Z. Zheng, Z.Q. Yang, S.L. Cai, L.T. Zheng, Y. Xue, L. Chen, and J. Lin, Blood-brain barrier and blood-brain tumor barrier penetrating peptide-drug conjugate as targeted therapy for the treatment of lung cancer brain metastasis. Lung Cancer 196, 107957 (2024) [Google Scholar]
J. Hahn, S. Ding, J. Im, T. Harimoto, K.W. Leong, and T. Danino, Bacterial therapies at the interface of synthetic biology and nanomedicine. Nat. Rev. Bioeng. 2, 120–135 (2024) [Google Scholar]
G.E. Konecny and R.S. Kristeleit, PARP inhibitors for BRCA1/2-mutated and sporadic ovarian cancer: current practice and future directions. Br. J. Cancer 115, 1157–1173 (2016) [Google Scholar]

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.