BIO Web Conf.
Volume 8, 20172016 International Conference on Medicine Sciences and Bioengineering (ICMSB2016)
|Number of page(s)||10|
|Section||Session I: Medicine|
|Published online||11 January 2017|
A combination of gangliosides and nerve growth factor alleviates lipopolysaccharide-induced neuronal cells damage and its mechanism
1 College of Pharmaceutical Science, Zhejiang University of Technology, 310014 Hangzhou, China
2 Community Health Services Center of Mishixiang, Gongshu District, 310005 Hangzhou, China
a Corresponding author: firstname.lastname@example.org
Objective: To evaluate the effect of gangliosides (GM1) in combination with nerve growth factor (NGF) against neuronal cells damage evoked by lipopolysaccharide (LPS), and tries to uncover its probable mechanism. Methods: (1) Cell viability was measured using Methyl thiazolyl tetrazolium (MTT) method, which was also determined the optimum concentration of LPS for the damage models; meanwhile, cell morphology was observed by microscope. (2) The expression level of NF-κB was detected by RT-PCR. (3) Finally, NF-κB inhibitor pyrollidine dithiocarbamate (PDTC) was treated for the research of NF-κB pathway. Results: (1) MTT results shown that the LPS injury was dose-dependent, and 100nmol/L was selected as the optimum damage concentration. (2) Through the morphological observation, MTT and RT-PCR analysis, we found that GM1 and NGF both can protect cells against LPS injury; interestingly, combination of GM1 and NGF had a slighter LPS injury than GM1 administration alone. Moreover, the expression of NF-κB in combination group was lower than that in GM1 group, indicated that blockage of NF-κB pathway was better for cells living. Conclusion: Combination of GM1 and NGF has a better protective act on LPS injury than GM1 alone. The mechanism may have some connections with NF-κB pathways.
© The Authors, published by EDP Sciences, 2017
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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