Possibility of using combined transplantation of multipotent mesenchymal stromal cells and hepatic stellate cells to activate reparative liver regeneration in mature and old organism

¹ FSBEI HE Ural State Medical University Ministry of Health of the Russian Federation, 620028, Yekaterinburg, Russian Federation
² SAHI SO “Institute of Medical Cell Technologies”, 620026 Yekaterinburg, Russian Federation
³ Ural Research Institute of Phthisiopulmonology – branch of the Federal State Institution “National Medical Research Center of Phthisiopulmonology and Infectious Diseases” of the Ministry of Health of Russia, 620039, Yekaterinburg, Russian Federation

^* Corresponding author: makliu@mail.ru

Abstract

The aim of the study was to examine the effect of combined transplantation of multipotent mesenchymal stromal (MMSC) and hepatic stellate (HSC) cells on liver regeneration after resection. Research has been carried out on laboratory animals of mature and old age. After the subtotal resection of the liver, MMSC and HSC were introduced in the tail vein in the amount of 4 million cl/kg of body weight and 9 million cl/kg of body weight respectively. Evaluation of reparative liver regeneration was performed on the 1st, 3rd, 7th days after subtotal liver resection. Features of reparative liver regeneration in mature and old organism were revealed. In mature organism against the background of combined cell transplantation, regeneration activation is achieved by increasing cellular and intracellular regeneration mechanisms. In this case, the old organism responds to cell transplantation by activating only intracellular mechanisms. In both age groups, decreased mutagenesis and inhibition of programmed cell death against the background of MMSC cotransplantation and HSC were observed.