| Issue |
BIO Web Conf.
Volume 23, 2020
II International Scientific Conference “Plants and Microbes: The Future of Biotechnology” (PLAMIC2020)
|
|
|---|---|---|
| Article Number | 03005 | |
| Number of page(s) | 7 | |
| Section | Plant-Microbe Symbiosis, Including Natural and Artificial Symbiotic Systems | |
| DOI | https://doi.org/10.1051/bioconf/20202303005 | |
| Published online | 14 August 2020 | |
Studying the effect of tissue-specific expression of the K1 gene encoding LysM-receptor-like kinase on the development of symbiosis in peas
All-Russia Research Institute for Agricultural Microbiology, Laboratory of signal regulation, 196608 Saint-Petersburg, Russia
* Corresponding author: dol2helen@yahoo.com
To study the role of pea LysM receptor-like kinase K1 in the coordination of the infection process, starting in epidermis and nodule organogenesis in the root cortex of plants, during the development of rhizobium-legume symbiosis, the genetic constructs in which K1 gene was cloned under the control of tissue-specific promoter pLeEXT1 of tomato Lycopersicon esculentum extensin gene and the constitutive promoter of cauliflower mosaic virus (CaMV35S, cauliflower mosaic virus 35S) were obtained. During the transformation of the Nod- mutant line, the k1-1, with two types of constructs, the restoration of nodule formation was observed, which indicated the possible participation of K1 in the control not only early, but also later stages of symbiosis development in pea.
© The Authors, published by EDP Sciences, 2020
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