BIO Web Conf.
Volume 27, 2020International Scientific-Practical Conference “Agriculture and Food Security: Technology, Innovation, Markets, Human Resources” (FIES 2020)
|Number of page(s)||5|
|Published online||25 November 2020|
Archaea in the microbial community of the reindeer rumen in the Russian Arctic
“BIOTROF+” LTD, molecular genetic laboratory, St. Petersburg, 192284, Russia
2 North-west centre of interdisciplinary researches of problems of food maintenance, St. Petersburg, 196608, Russia
* Corresponding author: firstname.lastname@example.org
Archaea is the least studied group of the reindeer rumen microbiocenosis. Although the functional load performed by this group of microorganisms in the rumen is large. Methane-forming archaea play a key role in the process of anaerobic decomposition of organic substances, the formation of methane. This study for the first time analyzed the composition of the archaeal part of the microbial community of the reindeer rumen using the T-RFLP method from various regions of the Russian Arctic. As a result, it was found that according to the estimates of the number of archaea by quantitative PCR in the reindeer rumen in the winter-spring period, on average, 108 genomes/g of archaea were observed in individuals of the Yamalo-Nenets Autonomous District, and 109 genomes/in animals from the Nenets Autonomous District Archean. Thus, in the winter-spring period, a lower number of archaea in the rumen was observed in the Yamalo-Nenets Autonomous District. According to the results of the T-RFLP method, 44 to 134 phylotypes were detected in the archaeal community of the reindeer rumen, the Shannon index was 2.02–3.80. The lowest content (up to 11.10 %) of methanogenic archaea of the Methanomicrobia class (including the families Methanosarcinaceae and Methanocorpusculaceae) was revealed in the Nenets Autonomous District, while their presence in individuals of the Yamalo-Nenets Autonomous District reached 36.33 %. Interestingly, in adults of the Yamalo-Nenets and Nenets Autonomous Districts, a significant decrease in the representation of methanogenic archaea of the Methanomicrobia class was noted by 1.38 (P <0.05) and 2.70 times (P <0.01), respectively, compared with young individuals (up to 2 years).
© The Authors, published by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.