| Issue |
BIO Web Conf.
Volume 30, 2021
II International Symposium “Innovations in Life Sciences” (ILS 2020)
|
|
|---|---|---|
| Article Number | 05006 | |
| Number of page(s) | 7 | |
| Section | Fundamental and Applied Aspects of Biotechnology and Microbiology | |
| DOI | https://doi.org/10.1051/bioconf/20213005006 | |
| Published online | 22 April 2021 | |
Introduction of hyssopus officinalis l . into in vitro culture to optimize the conditions for obtaining callus tissues and microclonal propagation as a promising metod of innovative agrobiotechnologies
1
Belgorod State National Research University, Belgorod, Russia
2
Biotech company «Biocad», Moscow, Russia
3
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy
* Corresponding author: maslova@bsu.edu.ru
The sterilization process of plant explants of H. officinalis was optimized when introduced into an in vitro culture, the most effective sterilization modes, optimal sterilizing agents, their exposure time and concentration were selected. Callus tissues and mini-plants of H. officinalis were obtained in vitro and the most optimal nutrient media were determined both for microclonal propagation and for the induction of callus tissue H. officinalis, which can be further used for mass cultivation of cell and culture and obtaining safe bio-additives with active substances for livestock and crop production as a part of the development of modern agrobiotechnologies.
© The Authors, published by EDP Sciences, 2021
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