| Issue |
BIO Web Conf.
Volume 34, 2021
International Scientific Conference “Biologization of the Intensification Processes in Horticulture and Viticulture” (BIOLOGIZATION 2021)
|
|
|---|---|---|
| Article Number | 02005 | |
| Number of page(s) | 5 | |
| Section | Management of Genetic Resources and the Breeding Process of Creating Garden Crops and Grapes Based on Genotyping Methods, Precision Phenotyping, Bioinformatic and Digital Technologies | |
| DOI | https://doi.org/10.1051/bioconf/20213402005 | |
| Published online | 10 September 2021 | |
The using of gene-oriented SCoT markers in genotyping of the Apple genus (Malus Mill.)
Federal State Budget Scientific Institution «North Caucasian Federal Scientific Center of Horticulture, Viticulture, Wine-making», 39 str. 40 Let Pobedy, Krasnodar, 350901, Russia
* Corresponding author: ivstepanof@gmail.com
This paper discusses the aspects of optimization of the SCoT genotyping method for representatives of the genus Apple (Malus Mill.), including the orchard apple (Malus domestica). Special attention is paid to the methods of total DNA isolation from apple plant tissue, which is due to the sensitivity of multilocus marker systems to the quality of the nucleic acid preparation. On a sample of total DNA isolated from the leaves of the Golden Delicious cultivar, 18 SCoT markers were tested, from which 4 Malus Mill cultivars and species most promising for genotyping were selected.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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