BIO Web Conf.
Volume 34, 2021International Scientific Conference “Biologization of the Intensification Processes in Horticulture and Viticulture” (BIOLOGIZATION 2021)
|Number of page(s)||5|
|Section||Biotechnologies in the Organization of the Processes of Improvement and Reproduction of Horticultural Crops and Grapes|
|Published online||10 September 2021|
Polyploidy in tissues of plants in vitro of grape somaclones
All-Russian National Research Institute of Viticulture and Winemaking «Magarach», 31 str. Kirova, Yalta, Republic of Crimea, 298600, Russia
* Corresponding author: email@example.com
In vitro experimental plants obtained by clonal micropropagation of 9 grape somaclones of 5 original forms were the material for cytogenetic research. A biological microscope XSP-146TP was used for cytogenetic analysis. 823 cases of deviation from diploidy were observed in total. Significant tissue ploidy was observed in the meristematic tissue of in vitro plants of grape somaclones obtained by colchicine treatment of proembryogenic cells of various varieties. The significant direct correlation was found between the frequency of polyploidy in meristem tissues of in vitro plants and the number of chloroplasts in the stomata of grape somaclones. The reverse correlation was found between the frequency of polyploidy and the number of stomata on the leaf area. Somaclone No. 72, obtained as a result of regeneration from colchicinated proembryogenic cells of the Ruta grape variety and identified as a tetraploid (2n = 4x = 76), is recommended for use in the polyploid creation program.
© The Authors, published by EDP Sciences, 2021
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