Adjustment of culture media to optimize in vitro rhizogenesis of domestic plums of various origins

¹ Federal Research Center All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov, Crimean Experimental Breeding Station - branch of VIR, st. Vavilova, 12, Krasnodar region, Krymsk, 353384, Russia
² Krymsk Experiment Breeding Station of VIR, N.I. Vavilov All-Russian Institute of Plant Genetic Resources, Krymsk, Krasnodar Region, 353384, Russia

Abstract

This paper presents the results of the adjustment of 18 modifications of culture medium based on MS medium composition – Murashige and Skoog (1962) for in vitro rhizogenesis of 10 varieties of domestic plum. There were used 4 nutrient media with a full amount of macro- and microelements – MS₁ with a different combination of phytohormones, 1 medium without hormones – MSc, 13 media – MS₂ with half the amount of macronutrients, differing in hormonal composition. It was found that the maximum number of rooted microshoots from 21.2 to 52.2 % was on MS_2–8 medium, from 11.2 to 43.1 % on MS_2–5. The analysis showed that into the medium increases the percentage of microplants by 12-17 % comparing with the medium MS_2–5 and MS_2–7 without FA. The distinctiveness of nutrient media by the type of auxin (IAA, NAA, IBA) made it possible to clarify that IBA is the most optimal of the auxins, and the concentration of 1.7 mg/l is borderline for the growth reactions of plum varieties. It was revealed that _{in vitro} root formation depends not only on the compositions of growth substances in the nutrient medium, but also on the genotype of the variety.