| Issue |
BIO Web Conf.
Volume 41, 2021
The 4th International Conference on Bioinformatics, Biotechnology, and Biomedical Engineering (BioMIC 2021)
|
|
|---|---|---|
| Article Number | 05004 | |
| Number of page(s) | 6 | |
| Section | Biomedical Sciences and Engineering | |
| DOI | https://doi.org/10.1051/bioconf/20214105004 | |
| Published online | 22 December 2021 | |
Frequency of transovarial transmission of dengue virus in Aedes aegypti mosquitoes in endemic areas of dengue hemorrhagic fever in Ceper District, Klaten Regency, Central Java Province, Indonesia
Department of Parasitology, Faculty of Medicine, Public Health and Nursing Universitas Gadjah Mada, Indonesia
* Corresponding author: sitti-rahmah@ugm.ac.id
Klaten Regency is an area in Central Java Province with a high rate of endemic dengue hemorrhagic fever. This study aims to demonstrate a correlation between transovarial transmission and the incidence of dengue haemorrhagic fever in Ceper District, Klaten Regency. Aedes larvae and pupae were collected from January 2019 to March 2019 from various containers, using gamadotic and dipper tools, in Ngawonggo, Kajen, and Meger villages, which have high, medium, and low numbers of dengue cases, respectively. The larvae and pupae obtained were reared into adults. The presence of dengue virus in head squashes of 1-week-old female Ae. aegypti was identified based on an immunocytochemical assay using DSSE10 monoclonal antibody, made at Universitas Gadjah Mada as a primary antibody. The frequency of transovarial transmission of dengue virus (TIR) in Ae. aegypti in the three villages was 35.7%. The highest frequency occurred in Ngawonggo Village, at 40 %, and the lowest in Meger Village, at 28%, while the frequency in Kajen Village was 36.9 %. This study proves that there is a strong correlation (r = 0.99664) between transovarial transmission and the incidence of dengue hemorrhagic fever in Ceper District, Klaten Regency, Central Java.
Key words: Dengue hemorrhagic fever / Aedes aegypti / immunocytochemical assay / transovarial / Klaten
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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