Mortality Effect of Bintaro (Cerbera manghas L.) Seed Extract Against Aedes aegypti (Diptera: Culicidae) Mosquitoes

Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Malang, Jl. Raya Tlogomas 246 Malang 65144, Indonesia

^* Corresponding author : tutut.indria@umm.ac.id

Abstract

Bintaro (Cerbera manghas L.) seeds contain phytochemical compounds like cerberin, flavonoid, steroid, tannin, and saponin. These compounds may act as a natural eradication of Aedes aegypti L. in the adult stage or are called natural adulticides. This study aimed to determine the effectiveness of Bintaro seed extract on the mortality of Ae. aegypti mosquitoes. This experimental research uses the post-test-only control group design. There were seven Bintaro seed extract concentrations as treatment, namely 300ppm, 350ppm, 400ppm, 450ppm, 500ppm, 550ppm, and 600ppm. The control treatment used ethanol (96%). The research sample was 480 mosquitoes aged 3-5 days (for three repetitions). Each treatment uses 20 mosquitoes in a Duran bottle 250 mL. The data were processed using SPSS type 22 with normality and homogeneity tests, then analyzed using the Kruskal-Wallis test to determine differences in effective concentrations on mosquito mortality. The results showed that Bintaro seed extract at a concentration of 600ppm was the most effective on the mortality of Ae. aegypti mosquitoes but not significantly different from the other concentrations. While the results of the LC50 probit regression analysis were 453.071 ppm, and the LC90 value was 1737.760 ppm.