Utilization of Bintaro (Cerbera manghas L.) leaves extract as an Aedes aegypti L. mosquito killer: How is its effectiveness?

Department of Biology Education, Faculty of Teacher Training and Education, Universitas Muhammadiyah Malang, Jl. Raya Tlogomas 246 Malang 65144, Indonesia

^* Corresponding author : tutut.indria@umm.ac.id

Abstract

Aedes aegypti L. Mosquito is the only vector of Dengue Hemorrhagic Fever (DHF). The chemical-based control agents have a negative effect if used for a long time. Therefore, this research focused on finding the natural agent that may act as an adulticide, like Bintaro (Cerbera manghas L.). Bintaro leaves contain active compounds such as cerberin (one of the alkaloids), flavonoids, tannins, steroids, and saponins that have a mortality effect on mosquitoes. The study was to determine the effectiveness of Bintaro leaves extract as an adulticide against Aedes aegypti mosquitoes. This experimental research uses the posttest-only control group design and uses Ae. Aegypti mosquito aged 3-5 days as the sample. There were seven different concentrations of Bintaro leaves extract as a treatment, namely 300 ppm, 350 ppm, 400 ppm, 450 ppm, 500 ppm, 550 ppm, and 600 ppm. The method used in this research is using a 250 ml Duran bottle. The results of the study showed that the Bintaro leaves extract at a concentration of 600 ppm was more effective against the mortality of Ae. Aegypti, but not significantly different from the 400 ppm, 450 ppm, 500 ppm, and 550 ppm treatments. And the LC50 probit regression analysis was 455.602 ppm, and the LC90 value was 1735.956 ppm.