BIO Web Conf.
Volume 41, 2021The 4th International Conference on Bioinformatics, Biotechnology, and Biomedical Engineering (BioMIC 2021)
|Number of page(s)||6|
|Section||Drug Development and Nutraceutical|
|Published online||22 December 2021|
Potential of Nanoparticles Chitosan for Delivery pcDNA3.1-SB3-HBcAg
Biomedical Field, Nursing Study Program, STIKES Yarsi Mataram, West Nusa Tenggara, 83361, Indonesia
2 Midwifery Study Program, STIKES Yarsi Mataram, West Nusa Tenggara, 83361, Indonesia
3 Nursing Study Program, STIKES Yarsi Mataram, West Nusa Tenggara, 83361, Indonesia
4 Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, University Gadjah Mada, Yogyakarta, 55281, Indonesia
* Corresponding author: firstname.lastname@example.org
Hepatitis B virus (HBV) is a DNA virus that causes hepatitis in humans. This study aims to prepare a Hepatitis DNA vaccine. he optimized base sequence of the SB3-HBcAg gene was derived from the nucleotide base sequence of the Hepatitis B core antigen B3 HBcAg subgenotype, and then Cloning of the pcDNA3.1-SB3-HBcAg has been successfully performed on E. coli DH5α and confirmed by PCR, restriction analysis and sequencing. The propagated plasmids were prepared as DNA-chitosan complex and physiochemical characterized using Particle Size Analyzer. Complex with a 4:1 (wt/wt) ratio of DNA with 0.04% concentration and chitosan have a mean diameter of 231.7 nm and zeta potential +12.3 mV and the value of Cytotoxicity Assay 80-90% as compared to the untreated cells that used as negative control, so it can be concluded that nanoparticles chitosan has good potential as a carrier agent for pcDNA3.1-SB3-HBcAg.
Key words: A codon optimization / Chitosan nanoparticles / HBcAg / Hepatitis B virus / Delivery System
© The Authors, published by EDP Sciences, 2021
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