BIO Web Conf.
Volume 41, 2021The 4th International Conference on Bioinformatics, Biotechnology, and Biomedical Engineering (BioMIC 2021)
|Number of page(s)||6|
|Section||Drug Development and Nutraceutical|
|Published online||22 December 2021|
Potential of Nanoparticles Chitosan for Delivery pcDNA3.1-tat
Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, University Gadjah Mada, Yogyakarta, 55281, Indonesia
2 Department of Pathology, Faculty of Veterinary Medicine, University Gadjah Mada, Yogyakarta, 55281, Indonesia
3 Biomedical Field, Nursing Study Program, STIKES Yarsi Mataram, West Nusa Tenggara, 83361, Indonesia
* Corresponding author: firstname.lastname@example.org
The development of Jembrana disease vaccine is importance to prevent the loss of Bali cattle industry in Indonesia. This study aims to prepare a Jembrana DNA vaccine. The data Tat protein sequences gained from NCBI and the consensus process has been finished by the MultAlign program, and then Cloning of the pcDNA3.1-tat has been successfully performed on E. coli DH5α and confirmed by PCR, restriction analysis and sequencing. The propagated plasmids were prepared as DNA-chitosan complex and physiochemical characterized using Particle Size Analyzer. Complex with a 1:2 (wt/wt) ratio of DNA and chitosan have a mean diameter of 268.5 nm and zeta potential +25.1 mV and the value of Cytotoxicity Assay 80-90% as compared to the untreated cells that used as negative control, so it can be concluded that nanoparticles chitosan has good potential as a carrier agent for pcDNA3.1-tat.
Key words: A consensus sequences / Chitosan nanoparticles / JDV Tat protein / Jembrana disease virus / Delivery System
© The Authors, published by EDP Sciences, 2021
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