| Issue |
BIO Web Conf.
Volume 45, 2022
68th Scientific Conference with International Participation “FOOD SCIENCE, ENGINEERING AND TECHNOLOGY – 2021”
|
|
|---|---|---|
| Article Number | 02003 | |
| Number of page(s) | 5 | |
| Section | Food Chemistry, Microbiology and Biotechnology | |
| DOI | https://doi.org/10.1051/bioconf/20224502003 | |
| Published online | 04 February 2022 | |
In vitro simulation of the gastrointestinal tract environment and its interaction with probiotic lactobacilli
1 Department of Microbiology, University of Food Technologies, Plovdiv, Bulgaria
2 Department of Biotechnology, University of Food Technologies, Plovdiv, Bulgaria
* Corresponding author: d_blazheva@uft-plovdiv.bg
The harshest conditions of the human gastrointestinal tract were simulated in order to study probiotic bacteria in their intended environment. Eight Lactobacillus strains were cultivated in MRS broth with added bile in different concentrations and their growth was monitored as optical density. The gathered data was used to determine the MIC of bile for each strain. The recovery of the strains in MRS broth after 3 h in simulated gastric juice solution (pH 1.8, 5000 U/cm3 pepsin) was investigated. Lactobacillus gasseri S20 exhibited the best survival rate and reached OD 600 0,490, while Lactobacillus acidophilus S11 could not survive the conditions of the stomach (OD 600 0,076).
© The Authors, published by EDP Sciences, 2022
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