BIO Web Conf.
Volume 60, 20232022 4th International Conference on Biotechnology and Food Science (BFS 2022)
|Number of page(s)||9|
|Section||Biochemical Application and Genetic Engineering|
|Published online||11 May 2023|
The Construction of Triple-Deleted Mutant M18ΔUMS as Cell Factory in Order to Improve PCA Production
School of Life Sciences and Biotechnology, Shanhghai Jiao Tong University, Shanghai 200240, China
* Corresponding author: Yangmike24@gmail.com
Phenazines are secondary metabolites chiefly known for their broad-spectrum anti-microbial property. Phenazine-1-carboxylic acid (PCA) produced by Pseudomonas aeruginosa, a gram-negative bacterium, is an effective biocontrol agent against a number of plant-related pathogens. This experiment aimed to increase the production of PCA by deleting genes UTR, phzM and phzS in Pseudomonas aeruginosa M18, thus rendering the new strain more suitable for commercial use. The triple-deleted mutant M18ΔUMS shows significantly increased performance in PCA production compared to wild-type M18 strain. The new strain should be more applicable for commercial usage. However, M18ΔUMS exhibits a decreased growth rate compared to M18, indicating a inhibitory effect caused by the excess PCA.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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