BIO Web Conf.
Volume 60, 20232022 4th International Conference on Biotechnology and Food Science (BFS 2022)
|Number of page(s)||4|
|Section||Biochemical Application and Genetic Engineering|
|Published online||11 May 2023|
Loss of Setd2 Induces the Upregulation of Genes Related to Akt/Mtor Signaling Pathway
1 College of Life Sciences Sichuan University Chengdu China
2† Northwest A&F University China
Patients with polycystic kidney disease (PKD) have a high risk of developing renal cell carcinoma (RCC). SET domain–containing 2(SETD2) is the only molecule known to regulate lysine trimethylation (H3K3me3) of histone H3 in human tissue, and SETD2 is identified as a tumor suppressor in ccRCC. Although there are some studies revealing some mechanism about PKD developing ccRCC, the underlying mechanism remains largely reported. We collected the Kidney samples from SETD2 conditional knockout mice described before (Rao, 2021) and detected the expression levels of some important genes related to Akt/mTOR signaling pathway. Besides, we found that SETD2 is closely related to Akt/mTOR signaling pathway and can be regulated by Western blot analysis, qRT-PCR and immunofluorescence. For clinical translation, the cross-talks between SETD2 and Akt/mTOR signaling may provide a potential strategy to prevent tumorigenesis in patients with ccRCC therapy.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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