BIO Web Conf.
Volume 65, 2023EBWFF 2023 - International Scientific Conference Ecological and Biological Well-Being of Flora and Fauna (Part 2)
|Number of page(s)||8|
|Section||Preventive Healthcare and Urban Ecology|
|Published online||04 September 2023|
Molecular screening of oxacillinases beta-lactamase among gentamicin-resistant Escherichia coli isolates
University of Kufa, Faculty of Science, Department of Biology, Iraq
* Corresponding author: firstname.lastname@example.org
Expansion of β-lactamases in Gram-negative rods has been documented as most severe threat to the management of infectious diseases. The ever-increasing use of antibiotics with the evolution of intrinsic and acquired resistance has led to the development of resistance mechanism in Gram-negative rods contributing to the expansion of several multi-drug resistance epidemics in hospital environment. So this study aimed to investigate genes responsible for Oxacillinases production among gentamicin- resistant Escherichia coli isolates. The results showed that out of 573 specimens, 270 (56.13%) showed bacterial growth versus 264(46.07%) showed no bacterial growth 309(53.92%), among 573 clinical specimens 102(17.80%) were male patients while 471(82.19%) were female patients. According to result of the vitek-2 system recorded 110 isolates as E. coli. However result of gentamicin susceptibility demonstrated that 29 (26.36%) E. coli isolates were resistance to gentamicin compared with 39 (35.45%) and 42 (38.18%) of isolates were intermediate and sensitive to this antibiotic respectively. Results of antibiotic susceptibility showed that the highest bacterial resistance was Tobramycin 27(93.1%) and Ciprofloxacin 29(100 %),while Nitrofurantoin 3(10.3%) had the least resistance. Results of polymerase chain reaction (PCR ) amplification showed that OXA, OXA-1 and OXA-9,OXA-10 were 29(100%),26/(89.65%),29(100%) and 20(68.96%) respectively While OXA-2 did not detect.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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